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Enzyme - pH

Effect of pH on rate of enzyme reaction - Leaving Cert Biology

QuestionAnswer
What should you be aware of when doing this experiment? That there is Hydrogen Peroxide (H2O2) involved, therefore safety goggles and gloves must be worn due to its Harmful and Irritating nature to the skin.
What is the 1st step in pH enzyme test? Place some pH buffer solution 4 into a graduated cylinder (pH 4 buffer ensures that the solution remains at pH 4).
Step 2 of the pH enzyme experiment? Use a dropper to add one drop of washing up liquid to the pH buffer 4 solution in the graduated cylinder.
What is the reason for adding washing up liquid in this experiment? It will trap any volume of oxygen (O2) produced in the reaction, so that it can be measured to see which pH is most/least effective.
The 3rd step of the pH experiment? Blend some celery in a blender and then filter solution into a beaker using coffee filter paper.
Fourth step of the experiment? Add some of the filtrate to the graduated cylinder. (Celery contains the enzyme that is being used in the experiment: Catalase).
What is the Fifth step of the pH experiment? Add Hydrogen Peroxide (H2O2) to another graduated cylinder. H2O2 is the substrate that breaks down the enzyme catalase.
6th step of the pH experiment? Stand both graduated cylinders in a water bath at 25 degrees Celcius.
The Seventh step of the pH experiment? Remove the two graduated cylinders from the water bath and pour the H2O2 into the graduated cylinder with the blended celey in.
Eighth step of the pH experiment? Note and record the volume of foam after 2 minutes.
How do you calculate the amount of foam produced in the reaction? You subtract the original volume of the liquid in the graduated cylinder from the volume at the top of the foam after 2 minutes. The foam indicates the rate of reaction.
What happens after the you caculate the rate of reaction? You then repeat steps 1-9 using pH buffers 7, 10 and 13. A graph of results is then placed in a results graph.
Created by: AW117
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