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Enzymes

Immobilised, denatured etc

QuestionAnswer
What is an enzyme? biological catalysts that have very specific shapes
What are enzymes made of? Globular proteins
What is a catalyst? It speeds up rates of reactions without being used up in the reaction
Name the two theories surrounding how enzymes work The Lock and Key Theory and the Induced fit theory
What is an easy way to identify if something is an enzyme? They all end in 'ase'
Do enzymes promote catabolic or anabolic reactions? Both
Give an example of an anabolic enzyme and what is it involved in? ***DNA polymerase- involved in making DNA during DNA replication
Name the enzyme that works on protein and where does it work? Protease works in the stomach
What does protein break down into in catabolic reactions? Amino acids
Name the enzyme that works on lipids and where does it work? Lipase works in the small intestine
What do lipids break down into in catabolic reactions? 1 glyceroll and 3 fatty acids
Name the enzyme that works on starch and where does it work? Amylase works in the small intestine and mouth
What does starch break down into in catabolic reactions? Maltose
Name two factors that effect enzyme action Temperature and pH
What does denature mean? when the active site changes shape and the substrate will no longer fit
Describe how the lock and key theory works lock=enzyme, key=substrate, keyhole=active site --> the substrate and enzyme fit together like a lock and key
What is an immobilised enzyme? An enzyme that has been fixed or attached to another enzyme or an inert material (eg sodium alginate)
Can the shape of an enzyme be changed when immbolised? Yes
Name two uses of enzymes in industry Fizzy drinks and toffee making
Describe how fizzy drinks are made Glucose is converted to fructose using glucose isomerase
Describe how toffee is made lactose is converted into glucose and galactose using lactase
Describe how the induced fit theory works 1. substrate is complimentary to active site, but doesn't fit perfectly 2. active site slightly changes to fit shape of substrate 3. the enzyme remains the same while the substrate becomes a product
What is a substrate? What is involved in the reaction
What is an active site? Where the reaction takes place
Created by: sjbuzb
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