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DNA Extraction Exp.

QuestionAnswer
Step 1 add salt, washing up liquid and water to a graduated cylinder
Step 2 chop up piece of onion
Step 3 mix chopped onion and salt-detergent solution in a beaker
Step 4 place in a water bath at 60deg for 15 minutes
Step 5 Place in an ice-bath for 5 minutes
Step 6 Blend the solution for no more that three seconds
Step 7 Filter the solution for through filter paper and a funnel
Step 8 Put some solution in a testube and add a few drops of protease enzyme
Step 9 Gently pour icecold ethanol ontop of the onion solution - dna should appear at the interface between the two solutions
Step 10 The Dna can be seen on a glass rod
Why did you use washing up lqd. to breakdown membranes
Why did you use freezer-cold ethanol To separate the DNA
Where in PLant cells in DNA found in chloroplasts, mitochondria and nuclei
Why did you blend the solution to break down the cell walls - for three seconds only
Why is sodium chloride used to clump DNA and remove proteins
Why is protease used to break down the proteins that secure DNA together
Why did you chop the onion to increase its surface area and braeak cell walls
Why was the mixture heated to inactivate enzymes
Why should you only blend the mixture for three seconds otherwise the DNA would get shredded
Created by: serrraphina
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