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Bio Unit 3 Sec 3 #1

Bio Unit 3 Sec 3 #1: Lec 24 : Tools of Biotechnology

QuestionAnswer
The application of scientific techniques to modify and improve plants, animals, and microorganisms to enhance their value and improve the quality of human life Biotechnology
Areas of biotechnology are _____, research, ______, and food science Medicine; Agriculture
Organisms that have been genetically modified by humans Transgenic Organisms
Transgenic organisms are created using _____ techniques Many
Bacteria's small, circular pieces od DNA separated from the main chromosomes are called: Plasmids
Plasmids can be bioengineered to contain desired ______ Genes
Bioengineered plasmids can be placed in _______ so they produce the product of the desired gene Bacteria
In Bioengineering, the _____ is removed from a desired organism so the desired genes can be isolated from the organisms ______ DNA; Genome
________ enzymes are used to pull the desired gene to be used in the bioengineering Restriction
Restriction Enzymes cut DNA at a particular _______ Sequence
Restriction Enzymes cut a desired gene out of an organisms _________ Genome
Restriction Enzymes cut a _______ to open it and create a space for the _______ gene Plasmid; Insertion
Restriction Enzymes leave exposed ______ at each cut site to allow the cut ends of the desired gene to ________ (connect) to the cut ends of the plasmid Bases; Anneal
The ________Chain Reaction rapidly increased the number of copies of ______ Polymerase; DNA
The Polymerase Chain Reaction (PCR) produces an exponential _____ in the amount of DNA and can make a billion copies by the 30th cycle Increases
The update of external DNA (plasmids) by bacterial cells is called: Transformation
Transformation allows the addition of _________ plasmid into the ________ so that the bacteria can produce the product of the desired gene Bioengineered; Bacteria
______ genes are used to show whether or not transformation was successful Marker
If transformation was successful, you can see the product of the marker gene with the _____eye Naked
An example of a marker gene is _______ Fluorescent Protein (PGI) and you can see the organisms fluoresce under UV lights Green
______ was the first cloned animal- sheep Dolly
It is _____ and _____ to clone humans, even though it could be done Unethical; Illegal
Human ______ is an example we studies of bioengineered bacterial cell Insulin
Created by: scarangelo
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