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Media

BACT-250

QuestionAnswer
Phenol Ethanol Agar (PEA) alcohol inhibits gm- selects for gm+
Mannitol Salt 7.5% salt selects for facultative halophile Staphlococcus phenol red differentiates S aureus acid end prdtcs w/ yellow
Bile Esculin Agar (BEA) Differentiates Enterococcus from Streptococcus Enterococcus produces iron salts-black media
Blood Agar Enriched(Fe) and Differential determines hemolysis
Alpha hemolysis in blood agar, partial breakdown of media. Greenish/cloudy
Beta-hemolysis in blood agar, complete breakdown of media. clear zones
gamma hemolysis in blood agar no breakdown of media, no zones.
Chemically Defined media exact composition is known, very expensive
Complex Media media varies by batch, but has either soy, beef or yeast extracts
Anaerobic or Reducing media reduces oxygen. Sodium thioglycollate bonds with oxygen to keep it unavailable to microbes
Bismuth Sulfate agar selects for Salmonella typhi agent of typhus. Inhibits gm+ and most gm-
Sabourands dextrose/glucose pH of 5.6, lower pH inhibits bacteria, encourages fungi
CNA Colistin & naladixic acid 2 antibiotics inhibit gm- Selects for Gram +
EMB eosin methylene blue selective and differential, Used in water treatment. Selects gm- indicates enteric organism if lactose is fermented Metallic Green Black
MacConkey S&D, used in hospitals Selects gm- with bile salts and crystal violet. indicates enteric organism if lactose is fermented pink
Centrimide selects for pseudomonas. flouresces under UV light if pseudomonas is present.
Created by: SonyaP
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