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ch.6 virus

cultivation of viruses

QuestionAnswer
in vivio in organisms
in vitro outside of living organisms
bacterial lawn dense layer of bacteriophages grown
importance of cultivation of animal virus identifying and diagnosing viruses, producing vaccines, research studies
viruses need these places to grow amniotic cavity, chorioallatoic membran or yolk sac
cell lines grow in lumps like tumors
HeLa tumor cells from Henerietta Lacks, cell line still alive today
how to detect virus hemaglutination assay, nucleic acid amplification tests, enzyme immunoassay
CPE'S cytopahtic effects, distinct observable abnormalities due to viral infection
effects of virus loss of adherence to the side, shrinking of nucleus, vacuoles in the cytoplasm, fusion of cytoplasmic membranes, formation of cytoplsmic syncytia, incluion bodies in the nucleus or cyto, cell lysis
hemaggulation assay detect certain types of virus, taken from plasma
hemmaggulatinins, surface proteins of spikes made by virus
nucelic acid amplification test detects unique nucleic acid sequence of virus
Enzyme Immunoassay, EIA antibodies attach to antigens, then enzyme attached to antigens, wash creates color change, similar to pregnancy tests
Created by: olivemaryjo
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