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ch.6 virus
cultivation of viruses
| Question | Answer |
|---|---|
| in vivio | in organisms |
| in vitro | outside of living organisms |
| bacterial lawn | dense layer of bacteriophages grown |
| importance of cultivation of animal virus | identifying and diagnosing viruses, producing vaccines, research studies |
| viruses need these places to grow | amniotic cavity, chorioallatoic membran or yolk sac |
| cell lines | grow in lumps like tumors |
| HeLa | tumor cells from Henerietta Lacks, cell line still alive today |
| how to detect virus | hemaglutination assay, nucleic acid amplification tests, enzyme immunoassay |
| CPE'S | cytopahtic effects, distinct observable abnormalities due to viral infection |
| effects of virus | loss of adherence to the side, shrinking of nucleus, vacuoles in the cytoplasm, fusion of cytoplasmic membranes, formation of cytoplsmic syncytia, incluion bodies in the nucleus or cyto, cell lysis |
| hemaggulation assay | detect certain types of virus, taken from plasma |
| hemmaggulatinins, | surface proteins of spikes made by virus |
| nucelic acid amplification test | detects unique nucleic acid sequence of virus |
| Enzyme Immunoassay, EIA | antibodies attach to antigens, then enzyme attached to antigens, wash creates color change, similar to pregnancy tests |