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Definitions
Random from 1st module
| Term | Definition |
|---|---|
| Decimal reduction time | time in which 90% of a bacterial population will be killed |
| Thermal death point | lowest temp which all bacteria in liquid will be killed |
| Thermal death time | minimum length for bacteria in liquid to be killed at given temp |
| Boiling | Not reliable for sterilization |
| Steam under pressure (autoclave) | YES sterilize |
| Pasteurization | Not reliable for sterilization |
| Direct Flaming and inceration | denatures, alters membranes and dehydrates |
| Hot-air sterilization | denatures, alters membranes, dehydrates at high temperatures |
| Dessication | involved removal of water, not reliable for sterilization |
| Lyophilization | also called freeze drying, not reliable for sterilization |
| Filtration | depending on pore size can be used for sterilization |
| ionizing | short wavelength and high energy emission (ex: x-rays) HIGHLY penetrable, reliable for sterilization |
| nonionizing | longer wavelength & lower energy emission (ex: UV light) lethal only at short wavelength to microbe, poorly penetrable |
| Thymidine dimers | can lead to mutations during DNA replication |
| Use dilution test | form of test to evaluate disinfectant, can check for growth |
| disk diffusion method | form of test to evaluate disinfectant, mesure zones of inhibition |