Busy. Please wait.

show password
Forgot Password?

Don't have an account?  Sign up 

Username is available taken
show password


Make sure to remember your password. If you forget it there is no way for StudyStack to send you a reset link. You would need to create a new account.
We do not share your email address with others. It is only used to allow you to reset your password. For details read our Privacy Policy and Terms of Service.

Already a StudyStack user? Log In

Reset Password
Enter the associated with your account, and we'll email you a link to reset your password.
Don't know
remaining cards
To flip the current card, click it or press the Spacebar key.  To move the current card to one of the three colored boxes, click on the box.  You may also press the UP ARROW key to move the card to the "Know" box, the DOWN ARROW key to move the card to the "Don't know" box, or the RIGHT ARROW key to move the card to the Remaining box.  You may also click on the card displayed in any of the three boxes to bring that card back to the center.

Pass complete!

"Know" box contains:
Time elapsed:
restart all cards
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.

  Normal Size     Small Size show me how

Bio Exam II

DNA Tech

PCR: Polymerase Chain Reaction - generates a lot of copies of DNA in short amount of time - Important when studying genes
PCR Components - Template DNA - primers - dNTPs ( dideoxynucleosides) A, T, C, G - Taq. polymerase
Process of PCR- done in thermal cycle to modulate temp. of mixture 1) denature: heat to 90 degrees C , breaks h-bonds in double helix 2) annealing: 40-65 degrees C, primers bind w/ complementary sequence in template stand 3) Extension: Taq. polymerase replicates DNA from 3'-5' end of primers. Strand become template
Gel Electrophoresis
Agarose Gel - polysaccharide gel - porous - passes electrical current through causing DNA to move from - to + end - Uses: confirms successful PCR, DNA sequencing, DNA profiling, Disease Diagnosis
Deoxyribonucleotide chain termination sequencing - 1st automated sequencing method - Sanger: won Nobel prize in 1980 -can sequence DNA fragments to about 1000 bp. - same process as PCR but w/ one major diff
Ingredients for Deoxyribonucleotide chain termination squencing - target dna - primer - DNA pol. -4 deoxyribonucleotides -4 dideoxyribonucleotides
Dideoxyribonucleotides - missing the 3' hydroxyl group making in responsible for chain termination because the phosphate cant bind
Process of Deoxyribonucleotide chain termination squencing - target DNA denatured - complementary strand synthesized - deoxyribonucleotide randomly incorporated - elongation stops (termination) at dideoxyribonucleotide
Polyacrylamide Gel - made from polymer - DNA fragments put in get and they migrate based on size (shorter strands move faster) - Detector reads bases as they migrate
Uses of sequencing - evolutionary relationships - determine loci & function - disease diagnosis
Applications - species diversity: know organisms in community, otherwise very hard to measure small & rare organisms - DNA bar coding: sequence of small region of genome to identify species
Gene Therapy - FDA approved gene therapy to cure type of genetic blindness; uses a retrovirus to insert correct genes into cell of retina
CRISPR - silence or insert genes
Created by: tennadeleta.