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Microbiolgy

QuestionAnswer
Staphylcoccus aureus can be differentiated from other staphylococcus species by using what test? Coagulase test; S. aureus is the only Staph that is coagulase positive.
What kind of media does Neisseria grow on? Chocolate agar or SBA
What is a good way to differentiate staph from strep species? Catalase test.
What reagent does the catalase test use? H2O2 (Hydrogen peroxide)
Describe the appearance of Neisseria species when using gram's stain. Gram negative diplococci
What does capnophilic mean? Carbon dioxide loving
What type of media is Mannitol Salt Agar (MSA)? Selective and differential for S. aureus.
Name the coagulase negative staph that is of particular concern to women of child bearing age. S. saprophyticus; causes UTI
Where would a viridians strep be found in an infected patient? usually in the blood (bactermia) or on heart valve replacements. Often can cause endocarditis.
What is a special nutrient requirement of viridians strep? Must have B6 and thiol
Which viridians strep causes dental caries? S. mutans
If S. bovis is found in the blood stream what does it indicate? Colon cancer.
What are the labs like for a person suffering from toxic shock? DIC (disseminated intravascular coagulation), decreased platelets, increased WBC (bands and metas), increased creatinine
Name some things that contribute to the virulence of Neisseria meningitidis. Pili, polysaccharide capsule, and endotoxin (lipopolysaccharide)
If a patient that was 19 years old came in complaining of a stiff neck, fever, and frontal headache; what would you suspect? How would you treat it? Neisseria meningitidis; Penicillin
What are the symptoms of food poisoning from S. aureus? Abd pain, nausea+vomiting, severe cramping, dehydration, and possible headache.
What is the most common alpha-hemolytic strep? Strep. pneumoniae
What enzyme does the Starch Hydrolysis test for? Amylase
Pseudomonas aeruginosa has what type of microscopic morphology? Gram negative bacillus
Explain what advantages a type of bacteria that produces catalase may have over a bacteria that does not produce catalase? A bacterium with catalase would be able to break down the by-products of Oxygen. These bacteria would be aerobic bacteria.
The process of heating a bacteria and then quickly cooling it to destabilize the cell membrane to integrate a DNA is called? and what technique did we use to accomplish this? The process is called Transformation and the technique is called Heat Shock.
How does ciprofloxin inhibit bacteria growth? It attacks the DNA gyrase to stop replication
How does Penicillin inhibit bacteria growth? It attacks the cell wall of the Bacteria
What enzyme is used to generate DNA from an RNA template in viruses? Reverse transcriptase (RT)
What chemokine receptor does HIV targets in macrophages and helper T cells? β-chemokine receptor (CCR5) in macrophages and α-chemokine receptor (CXCR4) in helper T cell
What do Influenza virus require in order to stick on cells? sialic acid
What is the DNA structure of the Influenza virus? Segmented genome structure
What is the common size for viruses? Usually 100- 200 nm
What is the common size for bacteria? 0.2- 20 um
When dealing with genes, what kind of transfer is "vertical transmission"? from parent to child
When dealing with genes, what kind of transfer is "horizontal transmission"? transfer of small pieces of DNA from one cell to another
Created by: Microbin