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Microbiolgy
| Question | Answer |
|---|---|
| Staphylcoccus aureus can be differentiated from other staphylococcus species by using what test? | Coagulase test; S. aureus is the only Staph that is coagulase positive. |
| What kind of media does Neisseria grow on? | Chocolate agar or SBA |
| What is a good way to differentiate staph from strep species? | Catalase test. |
| What reagent does the catalase test use? | H2O2 (Hydrogen peroxide) |
| Describe the appearance of Neisseria species when using gram's stain. | Gram negative diplococci |
| What does capnophilic mean? | Carbon dioxide loving |
| What type of media is Mannitol Salt Agar (MSA)? | Selective and differential for S. aureus. |
| Name the coagulase negative staph that is of particular concern to women of child bearing age. | S. saprophyticus; causes UTI |
| Where would a viridians strep be found in an infected patient? | usually in the blood (bactermia) or on heart valve replacements. Often can cause endocarditis. |
| What is a special nutrient requirement of viridians strep? | Must have B6 and thiol |
| Which viridians strep causes dental caries? | S. mutans |
| If S. bovis is found in the blood stream what does it indicate? | Colon cancer. |
| What are the labs like for a person suffering from toxic shock? | DIC (disseminated intravascular coagulation), decreased platelets, increased WBC (bands and metas), increased creatinine |
| Name some things that contribute to the virulence of Neisseria meningitidis. | Pili, polysaccharide capsule, and endotoxin (lipopolysaccharide) |
| If a patient that was 19 years old came in complaining of a stiff neck, fever, and frontal headache; what would you suspect? How would you treat it? | Neisseria meningitidis; Penicillin |
| What are the symptoms of food poisoning from S. aureus? | Abd pain, nausea+vomiting, severe cramping, dehydration, and possible headache. |
| What is the most common alpha-hemolytic strep? | Strep. pneumoniae |
| What enzyme does the Starch Hydrolysis test for? | Amylase |
| Pseudomonas aeruginosa has what type of microscopic morphology? | Gram negative bacillus |
| Explain what advantages a type of bacteria that produces catalase may have over a bacteria that does not produce catalase? | A bacterium with catalase would be able to break down the by-products of Oxygen. These bacteria would be aerobic bacteria. |
| The process of heating a bacteria and then quickly cooling it to destabilize the cell membrane to integrate a DNA is called? and what technique did we use to accomplish this? | The process is called Transformation and the technique is called Heat Shock. |
| How does ciprofloxin inhibit bacteria growth? | It attacks the DNA gyrase to stop replication |
| How does Penicillin inhibit bacteria growth? | It attacks the cell wall of the Bacteria |
| What enzyme is used to generate DNA from an RNA template in viruses? | Reverse transcriptase (RT) |
| What chemokine receptor does HIV targets in macrophages and helper T cells? | β-chemokine receptor (CCR5) in macrophages and α-chemokine receptor (CXCR4) in helper T cell |
| What do Influenza virus require in order to stick on cells? | sialic acid |
| What is the DNA structure of the Influenza virus? | Segmented genome structure |
| What is the common size for viruses? | Usually 100- 200 nm |
| What is the common size for bacteria? | 0.2- 20 um |
| When dealing with genes, what kind of transfer is "vertical transmission"? | from parent to child |
| When dealing with genes, what kind of transfer is "horizontal transmission"? | transfer of small pieces of DNA from one cell to another |
Created by:
Microbin
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