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micro lab 125
bio 125
| Question | Answer |
|---|---|
| what are the 4 lens | red (scanning) 4x yellow (low power) 10x blue (high power) 40x white (oil immersion) 100x |
| What is the optical part of the microscope? | objective lens |
| what is the function of the condenser? | allow more or less light in |
| Why is the function of the iris diaphragm.? | controls light intesity |
| What part of the human eye does the iris diaphragm compare too. | iris |
| why is oil used between the specimen and the oil immersion objective? | increases power of the microscope. keeps light in by bending it. |
| Define parfocal | lens that stays in focus when magnification focal length is changed |
| define resolution | shortest distance between two points on a specimen that can be distinguished. |
| define working distance | between lens and slide |
| define magnification | refined power (enlarge object) |
| define field of view? | observable area seen though lens |
| define ocular | lens that magnify 10 times |
| define revolving nosepiece | rotates to change from one objective to another. |
| define objectives? | lenses of 4 difference magnification |
| define slide holders | spring-loaded portion allows placing the slide in the mechanical stage where it is tightly held |
| define diaphragm lever | opens and closes diaphragm to control amount of light striking objects |
| define condenser | condenses light waves into pencil-shaped cone, thereby pre venting escape of light waves; also controls amount of light striking object. |
| define condenser adjustment | raises and lowers condensers |
| define mechanical stage | allows the slides to be moved |
| define mechanical stage controls | moves the slide on two horizontal planes |
| define base | supports the entire microscope |
| define arm | used in carrying the microscope |
| define coarse adjustment | moves stage up and down very slowly for approximate focusing |
| define fine adjustment | moves stage up and down for purposes of definitive focusing |
| define body tube | supports upper portion of microscope |
| at which point (power of magnification) can bacterial morphology ne determined? why? | 1000x to clearly see shape/ inside structures |
| what type of microscope would you use to observe a stained bacterial smear | compound light microscope |
| what type of microscope would you use to observe a virus | scanning electron microscope (SEM) |
| what type of microscope would you used as a diagnostic tool for tuberculosis | florescent microscope |
| what type of microscope would you use to observe the inside details of living microorganisms | light microscope |
| what is the equation for calculating final magnification of an image> | objective lens x ocular lens = final magnification. |
Created by:
xokitty17xo
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