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BioChem Quiz 2
BioChem Quiztwo except for glycolysis
| Question | Answer |
|---|---|
| The study of proteins in a cell is called: | proteomics |
| the technology used to study genes and proteins: (3 words) | recombinant dna technology |
| recombinant dna technology uses the____________process to make copies of DNA | cloning |
| Cloning uses____________as a vector which is found in bacteria. | Plasmid |
| A plasmid can be cleaved with a ____________(two words) in order to put a gene into it. | restriction endonuclease |
| Once a new gene is in a plasmid it is called a ________________vector | recombinant |
| _____________is the process of something being broken down and creates _________ and __________in the process and has a _____delta G. | Catabolism, ATP, NADH, negative |
| _________________is the process of building things up, which requires _________and __________, and has a _________delta G | Anabolism, ATP, NADH, positive |
| Anabolism has _________pathways and Catabolism has __________pathways, with common intermediates like ______________. | diverging, converging, acetyl COA |
| In translations you can have problems and get _______________(two words) which usually only differ at the _________base | degenerate codons, wobble |
| ____________is found only in trna and can bond with multiple nucleotides. | Inosine |
| In mutations you can have single changes which may or may not be expressed called _________(two words), or ______________(I) or _________________(D) which would cause a shift in the _____________________(two words) | point mutations, insertions, deletions, reading frame |
| In translation, the amino acid attached to the trna at the 3' end and you need ____________and ATP and two ___________are removed. Each of these are specific to one amino acid | synthetase, phosphate |
| In translation, the ______________(2 words)finds the met at the ___________site, which has a special ________ and needs both ____'s and _______to bind | initiation comples, P, tag, IF, GTP |
| In translation, after the initiation complex has bound, the next amino acid and trna binds to the __site forming a _________bond via ____________(two words). When this happen, __________occurs and the ribosome shifts, requiring both ____'s and _______. | A, peptide, peptidyl transferase, translocation, EF, GTP |
| Once the stop codon is recognized in translation, the chain __________ and leaves requiring _________'s and _________. | hydrolyzes, RF, ATP |
| The last stage of protein synthesis happens after the polypeptide chain has hydrolyzed which is ______________________(two/3 words) and ____________(two words) | post-translational modifications, protein sorting. |
| In translation, the mrna is read ___ prime to __prime, and the trna is built from the ___terminal to the ______terminal and starts at the _________amino. | 5,3, N, C, MET |
| In prokaryotes transcription and translation can occur | simultaneously |
| __________________(2words) can use RNA to make DNA in ____________. Here there it is less _________so there are more __________. | reverse transciptase, retrovirus, accurate, strains |
| In humans you can get _________which has two drugs that can be used against it, the first, ___________, resembles thymidine, and has an __________group. The second, _____________doesn't have a 3'________group. Both make it so next nucleotide wont add | HIV, Azidothymidine, Azido, Dideoxyinosine,OH |
| In transcrition, there are three phases: | initiation, elongation, termination |
| In transcription the rna is made _____prime to ______prime | 5, 3 |
| In transcription, the rna polymerase bonds to the __________or the ___________if in e.coli | promoter, Qsubunit |
| In transcription ____________bonds are formed when nucleotides are added | phosphodiester |
| RNA processing happens in the ___________, and a cap goes on the ______prime end which protects from ___________. A tail with _____'s is added on the ________end. ______are removed or ____________ and the rna goes to out of the cell. | nuclease, 5, nucleases,A, 3, introns, hydrolyzed |
| Free energy equals_____________, taking into account enthalpy and entropy, free energy under standard conditions , is ______________. the real drive in the cell, taking into consideration concentrations is the __________. | ∆G, ∆G°', ∆G' |
| ∆G is the difference between_________and _________, and is _________if spontaneous and ________if it needs energy | reactants, products,negative, positive |
| ∆G is the difference between __________and ______________. | enthalpy (∆H), entropy T∆S |
| ∆G' is the equation_______+____________. | ∆G°',RTlnQ |
| The measurement of energy in a phosphate bond is the _________(3words) | phosphate transfer potential |
Created by:
sbixby
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