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Coag. Negative Staph

CoNS Q/A

QuestionAnswer
What are the characteristics of Coagulase Negative Staphylococci (CoNS) when stained with Gram stain? The CoNS would be characterized as Gram-positive cocci in clusters, meaning they appear round and purple.
How would CoNS appear on a Blood Agar Plate in terms of hemolysis and colony? CoNS is non-hemolytic and grows in white, flat colonies.
Which media or testing method can be used to distinguish CoNS from Staphylococcus aureus? What would the CoNS positive look like? Mannitol Salt Agar; the colonies would appear pink because CoNS cannot ferment mannitol like Staph. aureus.
Which two chemical components can differentiate CoNS from Staphylococcus saprophyticus? Novobiocin (antibiotic) and Trehalose-Mannitol-Phosphatase agar (media)
What are some effects of the "slime" virulence factor produced by Coagulase Negative Staphylococci? -Harder to treat -Inhibits neutrophil chemotaxis and phagocytosis -Inhibits Vancomycin -Enhances adherence to plastic surfaces
In which body areas can CoNS be found as normal flora? Upper respiratory tract, skin, intestinal tract, mucous membranes
Created by: rowleea