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Coag. Negative Staph
CoNS Q/A
Question | Answer |
---|---|
What are the characteristics of Coagulase Negative Staphylococci (CoNS) when stained with Gram stain? | The CoNS would be characterized as Gram-positive cocci in clusters, meaning they appear round and purple. |
How would CoNS appear on a Blood Agar Plate in terms of hemolysis and colony? | CoNS is non-hemolytic and grows in white, flat colonies. |
Which media or testing method can be used to distinguish CoNS from Staphylococcus aureus? What would the CoNS positive look like? | Mannitol Salt Agar; the colonies would appear pink because CoNS cannot ferment mannitol like Staph. aureus. |
Which two chemical components can differentiate CoNS from Staphylococcus saprophyticus? | Novobiocin (antibiotic) and Trehalose-Mannitol-Phosphatase agar (media) |
What are some effects of the "slime" virulence factor produced by Coagulase Negative Staphylococci? | -Harder to treat -Inhibits neutrophil chemotaxis and phagocytosis -Inhibits Vancomycin -Enhances adherence to plastic surfaces |
In which body areas can CoNS be found as normal flora? | Upper respiratory tract, skin, intestinal tract, mucous membranes |