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Mycoplasma and Ureaplasma Smallest (0.3x0.8um) known free-living forms. No cell wall - trilaminar celll membrane. Cannot be gram stained. Resistant to beta-lactam. RNA and DNA can self-replicate.
Diagnosis of Mycoplasma and Ureaplasma Serology. Media: SP4, A8 agars and 10 B broth. Cholesterol is required of ureaplasma.
Mycoplasma hominis fried-egg CM and cause genital tract ulcers.
PPLO, SP4, A8 agars, and Shepards 10 B broth highly nutritious due to beef heart infusion, peptone supplemented with yeast extract, CMRL 1066 Medium and fetal bovine serum present in the medium. Arginine is required for goth
What are Amphotericin B, polymyxin B, and penicillin used for? inhibit faster growing contaminants
Where is Mycoplasma and Ureaplasma found? Only in humans in oropharynx, upper respiratory tract, and GI.
Transmission of Mycoplasma and Ureaplasma direct respiratory contact. They have adherence properties.
Mycoplasma pneumoniae Cause of 15-20% of pneumonia. Most common pneumonia in children. community acquired atypical (walking) pneumoniae
Cold Agglutinin Disease Antibodies are formed in response to infection M.pneumoniae. React with RBCs below body temp. and activates complement system
Lab diagnosis of M.pneumoniae Transport in M4. Direct detection with PCR. Fried egg morphology. Produces hydrogen peroxide. Cold Agglutinin Test: Any value >1:32 suggests a diagnosis
Treatment for M.pneumoniae macrolides, tetracycline. Resistant to beta-lactams.
Ureaplasma urealyticum 
 intense inflammatory reaction. Cause chorioamnionitis (placenta and amniotic fluids). Causes nongonococcal urethritis (NGU). Requires urea
Created by: Mirielles
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