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Biology chapt 14
Biology Chapter 14
Term | Definition |
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cloning | Production of identical copies. In organisms, the production of organisms with the same genes; in genetic engineering, the production of many identical copies of a gene. |
gene cloning | DNA cloning to produce many identical copies of the same gene. |
gene therapy | Correction of a detrimental mutation by the insertion of DNA sequences into the genome of a cell. |
transgenic organism | An organism whose genome has been altered by the insertion of genes from another species. |
recombinant DNA (rDNA) | DNA that contains genes from more than one source. |
vector | n genetic engineering, a means to transfer foreign genetic material into a cell—e.g., a plasmid. |
plasmid | Extrachromosomal ring of accessory DNA in the cytoplasm of prokaryotes. |
restriction enzyme | Bacterial enzyme that stops viral reproduction by cleaving viral DNA; used to cut DNA at specific points during production of recombinant DNA. |
DNA ligase | Enzyme that links DNA fragments; used during production of recombinant DNA to join foreign DNA to vector DNA. |
polymerase chain reaction (PCR) | Technique that uses the enzyme DNA polymerase to produce millions of copies of a particular piece of DNA. |
gel electrophoresis | Process that separates molecules, such as proteins and DNA, based on their size and electrical charge by passing them through a matrix. |
DNA fingerprinting also called DNA profiling | Is a technology that can identify and distinguish amoung indiciduals based on variations in their DNA. |
short tandem repeat (STR) sequence | Procedure of analyzing DNA in which PCR and gel electrophoresis are used to create a banding pattern; these are usually unique for each individual; process used in DNA barcoding. |
genetically modified organism (GMO) | Organism whose genetic material has been altered or enhanced using DNA technology. |
biotechnology products | Commercial or agricultural products that are made with or derived from transgenic organisms. |
gene pharming | Production of pharmaceuticals using transgenic organisms, usually agricultural animals. |
gene therapy | Correction of a detrimental mutation by the insertion of DNA sequences into the genome of a cell. |
ex vivo gene therapy | Gene therapy in which cells are removed from an organism, and DNA is injected to correct a genetic defect; the cells are returned to the organism to treat a disease or disorder. |
in vivo gene therapy | A process that takes place inside of a living oganism. |
genomics | Area of study that examines the genome of a species or group of species. |
human Genome Project (HGP) | Initiative to determine the complete sequence of the human genome and to analyze this information. |
structural genomics | Study of the sequence of DNA bases and the amount of genes in organisms. |
intergenic sequence | Region of DNA that lies between genes on a chromosome. |
repetitive DNA element | Sequence of DNA on a chromosome that is repeated several times. |
tandem repeat | Repetitive DNA sequence in which the repeats occur one after another in the same region of a chromosome. |
interspersed repeat | Repeated DNA sequence that is spread across several regions of a chromosome or across multiple chromosomes. |
functional genomics | Study of gene function at the genome level. It involves the study of many genes simultaneously and the use of DNA microarrays. |
DNA microarray | Glass or plastic slide containing thousands of single-stranded DNA fragments arranged in an array (grid); used to detect and measure gene expression; also called gene chips. |
comparative genomics | Study of genomes through the direct comparison of their genes and DNA sequences from multiple species. |
proteome | Sum of the expressed proteins in a cell. |
proteomics | Study of the complete collection of proteins that a cell or organism expresses. |
bioinformatics | Area of scientific study that utilizes computer technologies to analyze large sets of data, typically in the study of genomics and proteomics. |
homologous gene | Gene that codes for the same protein, even if the base sequence may be different. |