Help
Options
Didn't know it?
click below
click below
Knew it?
click below
click below
Don't Know
Remaining cards (0)
Know
retry
shuffle
restart
0:00
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.
Normal Size Small Size show me how
Normal Size Small Size show me how
Bio lab final
kms
| Question | Answer |
|---|---|
| Magnification vs. Resolution | Magnification enlarges a specimen in the field of view, whereas resolution is distinguishing two points as separate |
| Describe what a buffer does to keep the pH of solution stable | A buffer takes in excess H+ or OH- to maintain the pH of a substance |
| What is the H+ of a solution with a pH of 3? What is the OH of the substance? | H+ = 1*10^-3m OH=1*10^11m |
| Which test would you use to determine if starch was present in the solution? | Logol's Iodine |
| If starch is present in a solution, what color will the solution form when you add lugol's iodine? | a dark blue/ almost black |
| What is a negative result of lugol's iodine (starch not being present) | copper/ yellow / orangey color |
| What is the difference between an independent and a dependent variable ? | The independent variable is what is changed and tested in an experiment. The dependent variable is what's measured as a result of the independent variable |
| What test would you use to determine if amino acids are present in a mixture? | ninhdryin test |
| If filter paper turns purple when biuret's reagent is added, what is indicated? | amino acids are present |
| If a mixture turns purple when adding biuret's reagent, can you be sure that no proline is present ? | No because proline doesn't affect color change because it doesn't react with ninhydrin |
| Solution A: pH = 5.0 Solution B: [H+]=1*10^-6M T or F : Solution B contains more H+ than Solution A | false |
| Solution A: pH = 5.0 Solution B: [H+]=1*10^-6M T or F : Solution B contains 1*10^-8 OH ions | true |
| Solution A: pH = 5.0 Solution B: [H+]=1*10^-6M T or F: Solution A contains 1*10^-5 H+ | True |
| Solution A: pH = 5.0 Solution B: [H+]=1*10^-6M T or F: Solution B is more acidic than Solution A | false |
| Solution A: pH = 5.0 Solution B: [H+]=1*10^-6M T or F: Solution A and B differ in their H+ by a factor of 100 | true |
| The prescence of what should speed up the process of osmosis | aquaporins ( proteins that form water channels in the membrane) |
| What does benedicts reagent test for? | reducing sugars |
| What does a positive result of benedicts reagent look like? | yellow/ orange |
| what does a negative benedicts test look like? | blue |
| Which organelle is most abundant in the cells of the Elodea plant? What is the function? | Chloroplasts ; photosynthesis , se light energy to make glucose |
| Celery stalks that are immersed in fresh water for several hours become stiff and hard. Similar stalks left in a 0.15 M salt solution become limp and soft. From this we can deduce that | The fresh water is hypotonic and the salt solution is hypertonic to the cells of the celery stalks |
| If an animal cell was placed in a hypertonic solution, what is the best prediction of what would happen? | The cell will shrivel and possibly die due to loss of water by osmosis |
| Present in prokaryotes: cell wall | yes |
| Present in prokaryotes: central vacuole | no |
| Present in prokaryotes: chloroplasts | no |
| Present in prokaryotes: cytoplasm | no |
| Present in prokaryotes: DNA | yes |
| Present in prokaryotes: nucleus | no |
| Present in prokaryotes: nucleoid | yes |
| Present in prokaryotes: mitochondria | yes |
| Present in prokaryotes: plasmid | yes |
| Present in prokaryotes: plasmodesmata | no |
| Present in prokaryotes: ribosomes | no |
| Present in prokaryotes: peptidoglycan | yes |
| Present in prokaryotes: pasma membrane | yes |
| Present in animal cells : cell wall | no |
| Present in animal cells : central vacuole | no |
| Present in animal cells : chloroplasts | no |
| Present in animal cells : cytoplasm | yes |
| Present in animal cells : DNA | yes |
| Present in animal cells : nucleus | yes |
| Present in animal cells : nucleoid | yes |
| Present in animal cells : mitochondria | yes |
| Present in animal cells : plasmid | no |
| Present in animal cells : plasmodesmata | no |
| Present in animal cells : ribosomes | yes |
| Present in animal cells : peptidoglycan | yes |
| Present in animal cells : plasma membrane | yes |
| Present in plant cells: cell wall | yes |
| Present in plant cells: central vacuole | yes |
| Present in plant cells: chlorplasts | yes |
| Present in plant cells: cytoplasm | yes |
| Present in plant cells: DNA | yes |
| Present in plant cells: nucleus | yes |
| Present in plant cells: nucleoid | no |
| Present in plant cells: mitochondria | yes |
| Present in plant cells: plasmid | no |
| Present in plant cells: plasmodesmata | yes |
| Present in plant cells: ribosomes | yes |
| Present in plant cells: peptidoglycan | yes |
| Present in plant cells: plasma membrane | yes |
| How are cyanobacteria different from other prokaryotes | They are larger and perform photosynthesis |
| Define hypertonic : | Solute concentration is higher than that inside the cell |
| Define hypotonic: | Solute concentration is lower than that of solutes inside the cell |
| Define isotonic: | Solute concentration is the same |
| Define osmosis | net movement of water molecules from a high concentration to low concentration across a membrane |
| The magnification of an ocular is usually | 10x |
| Objective lens : 4x,10x,40x,60x,100x. What are the total magnifications? | 40x,100x,400x,600x,1000x |
| What is the relationship between magnification and field of view? | The greater the magnification, the smaller the field of view. This is because higher magnifications show only tin portion of the specimen at a higher magnification |
| Which objective provides the largest field of view? a.high b.100x. c.4x d. depends on the size of the stage | 4x |
| What test dye determines whether a fat is present in a mixture of substances containing water | Sudan IV |
| What does a positive Sudan IV test look like? | Red layer on top/ red orange |
| What does a negative Sudan IV test look like ? | clear |
| Exactly what part of the protein molecule is identified by a biuret test? | the peptide bond |
| What is the function of the central vacuole in plants? | To store nutrients, water, salts, and other things not currently being used by the cell |
| Name two plastids and their function | Chloroplasts: Contain chlorophyll / green pigment and help carry out photosynthesis Chromoplasts: Contain red/orange pigments Leucoplasts: clear and store lipids and other clear substances |
| What are amyloplasts | A certain type of leucoplast that store starch |
| What is the function of the revolving nosepiece/turret on a compound microscope | to change the objective lens |
| In the lab on large biological molecules, water was one of the solutions used in the biochemical tests that we did. What was the purpose of the water? | To serve as a neutral substance |
| endocytosis | entering cell |
| exocytosis | leaving cell |
| Describe how [H+] relates both to the acidity and alkalinity of solutions | The more H+ ions, the more acidic a solution i |
| If a benedicts test results in an orange color what type of molecule is present | reducing sugar/ carbohydrate |
| If a lugol's test results in a black result what type of molecule is present | carbohydrate/starch |
| If a Sudan IV test results in a red layer on top, what type of molecule is present? | lipid |
| If a biuret test results in a a purple color what molecule is present | Protein /peptide bonds are present |
| You are working on an experiment in lab and you need to have a solution with an exact pH of 5.3. You have two possible methods for determining pH: pH paper or a pH meter, Which method would you use to determine and adjust pH and why? | A pH meter because it gives a more accurate result |
| What is cyclosis | cytoplasmic streaming // orderly movement of chloroplasts in cytoplasm |
| Why do slugs die when salted | a higher solute concentration on the outside of the slug creates a concentration gradient where water moves out of the slug rapidly, causing the slug's cells to rapidly dehydrate |
| Why is biuret reagent and not ninhydrin used to determine whether protein is present in the solution? | The biuret test tests for peptide bonds (proteins) ninhydrin tests for free amino acids |
| Janus Green | visualize mitochondria |
| You are working in a lab and you need to examine the shape of the mouthparts of an insect that is 1 inch. in length. Which type of microscope would you use? | Dissecting scope |
| Which reagent visualizes mitochondria? | Janus green |
| One molecules reach a dynmic equilibrium on either side of a membrane they stop moving. true or false | false |
| What will happen if you place a plant cell in a beaker containing a hypertonic 30% NaCl solution? | plasmolysis |
| Plasmolysis | shrink and shrivel |
| cytolysis | swell and burst |
| List the scientific method | Ask a question, form a hypothesis, experiment, collect data, conclusion, repeat revise |
| What is the difference between resolving power and resolution? | Resolution is the ability to distinguish 2 points as separate and the resolving power is the power it is magnified at |
| What is the difference between cytoplasm and cytosol | Cytoplasm is contained by the plasma membrane and contains the insides of a cell, however cytosol is part of the cytoplasm that doesn't have an any organelles |
| Biology | study of life |
| Iris diaphram of microscope | adjusts amount of light striking object |
| nosepiece/turret | contains/rotates objective lenses |
| parfocal | in focus of one lens |
| resolving power | reveals detail |
| stereoscopic microscope | view large specimens |
| solution | homogeneous mixture of two solutions |
| solvent | liquid that dissolves the solute |
| solute | substance that dissolves |
| voltmeter | measures voltage difference between electrodes in an electrical circuit |
| sensing electrode | measures change in hydrogen concentration when acids or bases are added to a solution |
| reference electrode | what the sensing electrode is compared against |
| combination electrode | reference and sensing electrodes are housed within the same glass tube |
| 4 organic macromolecules | lipids, proteins, nucleic acids, carbohydrates |
| What is used as a negative control when performing substance presence tests | water |
| Benedict's test tests for | reducing sugars |
| What must you do in a benedicts test? | Heat the solution |
| After heating the solution in a benedicts test, what is a positive result | positive = orange/rusty color |
| Lugol's iodine tests for | starch |
| what color does lugol's iodine change a solution if starch is present? | blue/black |
| Lipids are hydrophobic or hydrophillic | hydrophobic |
| fats include | triglycerides, steroids, and phospholipids |
| are saturated fats solid or liquid at room temp? | solid |
| Nonpolar Sudan IV will appear as a | separate red layer and will not be present in polar portions |
| Amino acids are linked by | peptide bonds |
| What two tests can be used for proteins ? | ninhydrin test and biuret test |
| Ninhydrin tests for | free amino acids |
| biuret tests for | peptide bonds |
| What amino acid doesn't react with ninhydrin? | proline |
| failure to react with ninhydrin indicates | there are no free amino acids |
| Biuret tests test for presence of protein and peptide bonds, therefore this test is | qualitative, testing for presence ; cannot measure how much protein is present |
| When free amino acids react in a ninhydrin test, the solution changes to | purple color |
| What color is indicative of a protein in a biuret test? | purple |
| spectrophotometer | projects light through a solution and measures the amount of light transmitted through and amount of light absorbed by a solution |
| Prokaryotic cells lack | a nucelus and membrane enclosed organelles |
| Prokaryotic cells are part of Domains | Arachaea and Bacteria |
| all prokaryotic and eukaryotic cells are bound by a __________ ________________ | plasma membrane |
| cytoplasm is made up of | cytosol and organelles |
| cytosol is | liquid part of cell consisting of water and dissolved proteins |
| ribosomes are found in | all cells |
| ribosomes are resposnible for | protein synthesis |
| Plasmids function | protect bacteria in adverse environments by introducing antibiotic resistance or metabolized new nutrients |
| peptidoglycan | found only in bacteria; long polymers of sugar intersecting polypeptide chains of the cell wall |
| gram positive bacteria | have a thick peptidoglycan |
| gram negative bacteria | have a thin peptidoglycan |
| plasmodesmata | cytoplasmic strands transferring materials from one plant cell to another |
| middle lamella | thin sticky membrane that cements cell walls together (adjacent to eachother) |
| protoplast is the | living part of plant cell made up of nucleus and cytoplasm |
| anthocyanins | mineral salts, sugars, and plant pigments nonliving in cytosol |
| nucleus | control center |
| central vacuole | absorbs water and storage substances like anthocyanins |
| Most visible organelles are | plastids, a variety of vesicles |
| chlorplasts | store green pigment chlorphyll |
| chromoplasts | store orange/red pigments called carotenoids |
| leucoplasts | store lipids and other clear substances |
| amyloplasts | type of leucoplast that stores starch |
| cyclosis or cytoplasmic streaming | movement of chlorplasts in the cytoplasm |
| ________________ is commondly used to view plasmodesmata because of their thick cell walls | persimmon |
| nucleolus | produce small and large subunits of ribosomes |
| mitochondria | cellular respiration |
| Animals have 4 types of tissues: | epithelial , connective, muscle, and nervous |
| Methylene blue stains | nucleus blue |
| Red blood cells | erythrocytes : lack a nucleus; carry oxygen |
| white blood cells | leukocytes |
| lymphocytes | control cell mediated immunity |
| true or false platelets are not cells | true they are small sacs in blood that store clotting factors |
| three types of muscle cells | skeletal, cardiac, smooth |
| skeletal muscles are | long fibers with striations multinucleated |
| cardiac muscles are | branched cells 1-2 nuclei |
| smooth muscle is | spindle shaped 1 nucleus |
| neurons | numerous extensions called dendrites recieve incoming nervous impulses from the body axon : propogates impulses away |
| neuroglia | don't conduct nerve impulses; maintain motor neurons |
| cyanobacteria are special bacteria because | they have a large size and conduct photosynthesis |
Created by:
tiffanyritch
Popular Biology sets