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Immunoheme Exam 2

Which antigens are associated with the P system? P1
Which antigens are associated with the Globoside system P, Pk
What autoantibody is associated with Paroxysmal cold hemoglobinuria Anti-P
What lab test can confirm the presence of the Anti-P autoantibody Donath Landstiener test
What antibody is Mrs. Jay's Tumor (Anti-Tja) Anti-PP1Pk
What is the phenotype of a person presenting with the anti-PP1Pk antibody? p null
Give an example of a compound antigen Anti-f
Which Rh Antigen is the most immunogenic? D
Which I antigen is found in newborns? i^infant
What does the "I" stand for? Individuality
What is a compound antigen Epitopes with more than one Rh genes on the same chromosome
What is an antithetical antigen A pair or more than a pair of antigens that are coded by different alleles of the same gene (high incidence/ low incidence)
What is a high incidence (frequency) antigen? An antigen that almost everyone has (almost 100%)
Examples of high incidence antigens k, Lub
What is a low incidence (frequency) antigen? An antigen that is very rare, almost no one has it (<1%)
Examples of
What rbc morphology does one see in McLeod Syndrome? Why? Ansio/Polkiocytosis because Kx protein isn't made = reduced K antigen
Why does DTT neutralize Kell antigens Breaks the disulfide bond
Describe the significance of anti- "N" Mimics the N on GPA (glycophorin A) and reacts at 37 degrees celsius
What does HTLA high titer, low avidity
Kidd system is what kind of immunoglobulin? IgG
You need what to find Kidd enzyme enhancement
Why are HTLA antibodies usually clinically insignificant Low avidity makes the antibodies weak and can be diluted out so they have not been implicated in causing transfusion reactions and therefore are not "clinically significant"
KEL2 k (celano)
KEL3 Kpa (Penney)
KEL4 Kpb (Rautenberg)
KEL5 Ku (almost every RBC)
KEL6 Jsa (suttler)
KEL7 Jsb (matthews)
History of the Kell system Mrs. Kelleher; first to have hemolytic disease of the newborn
Function of Kell gene on erythrocytes Endothelian-d (converting enzyme) and vasocontrictor
Function of duffy on erythrocytes receptor for pro-inflammatory cytokines, transmembrane carrier
If you have no Kx protein do you express Kell? No
DTT, 2-ME, and AET function break disulfide bonds
Cause of McLeod syndrome no Kx protein
Results of Mclead syndrome ansio/polkiocytosis, decrease haptoglobin, increased reticulocytes, fragile, splenomegaly
Enzymes papain and ficin destroy Duffy, M, N
Enzymes papain and ficin enhance
An antibody demonstrating dosage would mean that Homozygous cells were stronger because you receive double the antigen, one allele from your mom and one from your dad
What p-value must be observed in the rule of 3? <=0.05 (gives a 95% confidence interval)
A DAT performed on a clotted sample stored at 4 degrees celsius may demonstrate in vitro complement attachment because a positive DAT demonstrates that the patients cells are coated with IgG antibodies and complement can be detected
Elution forcing off; acid elution's lower the ph and dissociates the antibody from the RBC membrane
Adsorbtion procedures remove autoantibody from serum to determine whether an underlying alloantibody exists
The purpose of additional procedures when working up a warm autoantibody is to identify potential underlying autoantibodies because warm autoantibodies agglutinate initially and mask underlying autoantibodies
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