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Lab 5

Formed from megakaryocytes residing in the bone marrow; released into peripheral blood platelets (thrombocytes)
how long to platelets circulate? what are they removed by? 9-12 days; liver and spleen
What are the 3 zones of a platelet? peripheral, sol-gel, organelle
Direct contact with blood, coagulation factors interact here, stickiness peripheral
microtubules and microfilaments; shape changes sol-gel
platelet metabolism organelle
What are the 3 main functions of a platelet? 1. maintain vascular epithelium 2. respond to vascular injury by producing platelet plug 3. participate in plasma coagulation and help form fibrin clot
Reference value for normal peripheral blood platelet count 150,000-450,000/mm^3 (ul)
How do you collect a satisfactory peripheral blood specimen for use in platelet counts? venous blood; EDTA anticoagulant
Red blood cell diluting pipette Capillary tube
Platelet diluting fluid 1% ammonium oxalate
Functions of platelet diluting fluid lyse RBCs, dilute out WBCs and platelets
Charge hemocytometer pipette at a 45 degree angle, tip at edge of coverglass, squeeze a drop of fluid and capillary action will draw it in
How do you prevent double counting of platelets? Don't count platelets on the bottom or right border, count them if at the top or left border
What is hemostasis The process which has evolved to prevent spontaneous bleeding and stop bleeding that has been initiated by trauma
vasoconstriction vascular
diversion of blood flow around an injured area vascular
activating platelets vascular
activating coagulation system when a clot is required vascular
maintenance of health vascular epithelial cells by releasing an endothelial growth factor platelet
stoppage of bleeding by forming platelet plug platelet
creation of stable hemostatic plug platelet
coagulation factors form fibrin coagulation
coagulation cascade coagulation
extrinsic and intrinsic pathways and common pathway coagulation
plasmin degrades fibrin and reestablishes blood flow fibrinolytic
promotes healing fibrinolytic
PT prothrombin time
used to monitor action of extrinsic system prothrombin time
APTT Activated partial thromboplastin time
evaluates function of intrinsic system APTT
what is the anticoagulant used in a one-stage PT sodium citrate, blue tube top
Whats the difference btwn serum and plasma serum is plasma without clotting proteins
what type of patient sample is used in a one-stage PT venous blood
typical reference value for PT 10-13 seconds
What reagent is used to preform a PT; what substances in it cause it to clot? thromboplastin - CaCl2 reagent; calcium and thromboplastin factor III



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