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Unit 7

Brucella species

QuestionAnswer
How does one acquire Brucella or "Undulant Fever"? It is acquired by direct contact with infected material such as animal carcasses, fetal membranes, vaginal discharge, mucous membranes, or unpasteurized milk. Meat packing plant workers are at risk.
What type of blood culture bottle is used to recover Brucella? What does it consist of? A biphasic blood culture bottle called Castaneda is needed, it contains a slant of enriched agar that is partially submerged in enriched broth. As the specimen is injected into the bottle the slant is covered.
What conditions must be met in order to grow Brucella? how long must it incubate for? Brucella is a slow grower so increased CO2 is needed and 21 days incubation to grow.
What symptoms does Brucella species cause? Local lymphadenopathy to formation of granulomas in major organs, bone or soft tissue
How are the Brucella species differentiated? All are oxidase positive so differentiated by increased CO2 requirements, susceptibility to bacteriostatic dyes such as thionine and basic fuchsin and by H2S production.
What is the most commonly recovered species of Brucella in our lab? What are its characteristics? Brucella melitensis is most commonly recovered, found in goats and sheep, does not require increased CO2, Urea variably, thionine (-), fuchsin (-), and H2S (-).
What special precautions should be taken when working with Brucella? Why? All suspected Brucellosis cases should be handled under a Biological Safety Cabinet or "hood" due to the aerosol mode of transmission.
What are four different species of Brucella? B. abortus (cows), B. suis (pigs), B. melitensis (sheep/goats), B. canis (dogs)
What does the term "undulating fever" describe? Normal temp. in the AM, high temp. in the PM
Created by: Cgover7