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Bacteriology
| Question | Answer |
|---|---|
| What does a bacterial cell membrane lack? | Sterols such as cholesterol |
| What are the functions of the bacterial capsule? | -Protection from dessication - Promoting virulence - Source of nutrition in starvation |
| What are the possible functions of the mesosomes? | -Compartmentalisation of DNA -Increase surface area for respiratory enzymes |
| What class of flagella are the fastest? | Monotrichous |
| What class of flagella are uniformly distributed across the cell? | Peritrichous |
| What are the functions of pili? | -conjugation -attachment sites for bacteriophage -attachment to other bacteria (biofilms) |
| What is the main component of the bacterial cell wall? | Peptidoglycan |
| What class of bacteria have a lipopolysaccharide layer? | Gram negative |
| How do we classify/test the difference in chemical complexity of the bacterial cell wall? | Gram staining |
| What is the primary stain in a gram stain? | Crystal violet |
| What is the mordant in the gram stain? | Iodine |
| What is the decolouriser in the gram stain? | Acetone |
| What is the counter stain in the gram stain? | Safranin |
| What are the three growth factors that bacteria need? | -Vitamins -Amino acids -Purines/Pyrmidines |
| What are autotrophs? | Use CO2 as their main source of carbon |
| What are heterotrophs? | Take in nutrients in preformed states |
| What are phototrophs? | Use light as their main source of energy |
| What are chemotrophs? | Gain energy by he oxidation of chemical compounds |
| What are psychrophile? (Give example of temp range) | Optimum temperature at the lower end of the temp scale and generally below 20 Celsius |
| What are mesohpiles? (Include temperature range) | These have similar ptimum temperatures to human cell and grow over a range of about 20-45 Celsius |
| What's classification of temperature must human pathogens be? | Mesophiles |
| What are Thermophiles? (Include temp range) | Thermophiles have an optimum temperature in the higher end of the temperature scale and have a range that is greater than 45 Celsius |
| What are obligate aerobes? | Must have oxygen present to grow |
| What are obligate anaerobes? | Must not have oxygen present to grow |
| What are facultative anaerobes? | Can grow with or without oxygen, but prefer its presence |
| What are aerotolerant bacteria? | Can grow equally well with or without oxygen present |
| What are microaerophiles? | Must have very low concentrations of oxygen (2-10%) |
| What are osmotolerant bacteria? | Can grow over a wide range of water activity/solute concentration |
| What are halophiles? | Require a high concentration of NaCl to grow |
| If conditions are favourable for bacterial growth, does sporulation take place? | No |
| In conjugation, what does the donor bacterium contain that the conjugating one does not? | F plasmid |