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Prokaryotes
Microbiology 3
Question | Answer |
---|---|
Compare and Contrast Eukaryotes and Prokaryotes: | Eukaryotes: Membrane bound organelles; large (80-100 micrometers); Single-Celled and Multicellular. Prokaryotes: No membrane-bound organelles; small (<10micrometers); Only Single-celled. |
Are Protozoans eukaryotes or prokaryotes? | Protozoans are Eukaryotes |
Are bacteria prokaryotes or eukaryotes? | Prokaryotes |
Explain genetic organization in Prokaryotes: | Genetic Material is in form of DNA. DNA is organized into singular circular chromosome located in nucleoid region. Genes are located on chromosomes and are codesfor proteins. |
What are 3 aspects of Cellular Compartmentalization in Prokaryotes? | Cytoplasm within the cell membrane; cell wall exterior to cell membrane; nucleoid is region with DNA. |
Protein synthesis occurs where (in prokaryotes) and is catalyzed by what? | In Prokaryotes, protein synthesis occurs in the cytosol and is catalyzed by ribosomes. |
What is the difference in bacterial ribosomes and most eukaryotic ribosomes? Why is this important? | Bacterial ribosomes are different (smaller) than eukaryotic ribosomes. This is important because we target bacterial ribosomes to kill cancer in chemotherapy. |
What are 2 targets of chemotherapy? | Bacterial ribosomes (because they are smaller than eukaryotes) and bacterial enzymes (they often have different enzymes) |
How do prokaryotes utilize energy? | Much like eukaryotes: oxidative phosphorylation. |
What is oxidative phosphorylation and where does it occur in prokaryotes vs. eukaryotes? | Aerobic (oxygen-requiring) synthesis of ATP (energy). Prokaryotes: occurs in cytosol. Eukaryotes: occurs in mitochondria. (enzymes found in mitochondrial membrane of eukaryotes are found in plasma membrane of prokaryotes) |
How is water balanced in cells? | Via Osmosis across cell membrane. |
What is an isotonic environment? | Same solute concentration inside/outside cell. |
What is a hypotonic environment? Will cell shrink or expand? | Lower solute concentration outside cell (Cell will expand). |
What is hypertonic environment? Will cell shrink or expand? | Higher solute concentration outside cell (cell will shrink). |
What function does cell wall serve in maintaining cell structure in hypotonic environment? | It helps prevent lysis (explosion of cell) in hypotonic environment. |
How do bacteria reproduce? | Via binary fission with mitosis. |
Are offspring of bacteria genetic clones or genetically different? | Genetic clones. |
What are 2 sources of genetic variability? Explain. | Mutation (accidental change in DNA) and Conjugation (exchange of DNA between bacteria) |
What are 2 theories of evolution of eukaryotes from prokaryotes? | Invagination of plasma membrane (accounts for organelles like endoplasmic reticulum and nuclear membrane). Endosymbiosis (accounts for mitochondria and chloroplasts). |
What does the gram stain differentiate? | If it is gram positive or gram negative - based on cell wall characteristics. (Positive is blue, negative is red) |
What are physical characteristics to look at when identifying bacteria? | Gram Stain, Morphology (shape: cocci, bacilli/rods, spiral), spore-forming, motility. |
What are 3 biochemical tests to perform when identifying bacteria? | 1) Based on ability to metabolize a particular chemical. 2) Citrate Test. 3) Lactose Test. |
What are serological tests based on? | Antigen- Antibody Reaction |
What are Nucleic Acid tests based on? | Genetic Makeup. |
What are 3 types of staining techniques? | 1) Simple Stain. 2) Negative Stain. 3) Differential Stain |
Explain Simple Staining: | Basic dye stains negatively charged bacteria. Heat fixation is required. (Methylene Blue) |
Explain Negative Staining: | Acidic (negative) dye is used. Leaves cell against a stained background and no heat fixation. |
Explain Differential Stain: | Differentiates between something. Gram staining: positive retains primary stain and appears blue/purple. Negative loses primary stain and retains secondary stain which is red/orange. |