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Microscope
Biology
| Question | Answer |
|---|---|
| How many microscopes should you lift at once? | One at a time. |
| How should you hold a microscope? | One hand on the arm and the other on the base. |
| Do not use your ____________ to clean the lenses, instead use _________ ___________. | Fingers, lens paper. |
| Always start on ____________ power. | Low. |
| Use the ___________ adjustment knob when on low power, then the _________ adjustment for better resolution. | Coarse, fine. |
| Why should you look at the microscope from the side when switching to high power? | To make sure the objective doesn't hit the slide. |
| True or False: Only use the coarse adjustment knob on high power. | False, fine adjustment knob. |
| Wet Mount Slide: 1. Place a ________ of water on the slide. 2. Place the __________ in the water. 3. Lower the cover slip on a -- degree angle to avoid ________ __________. | Drop, specimen, 45, air bubbles. |
| Magnification. | How an image is enlarged under the microscope. |
| Low power has ___________ magnification. | Lower. |
| High power has _____________ magnification. | Higher. |
| Total magnification formula. | Objective x eyepiece. |
| Resolution. | Amount of detail you can see of an image. |
| Field of view. | The diameter of light you see when looking through a microscope. |
| As magnification increases, the field of view ____________. | Decreases. |
| Low power has a _________ field of view. | Larger. |
| High power has a _____________ field of view. | Smaller. |
| True or False: Low power is darker. | False, brighter. |
| True or False: High power is darker. | True. |
| Depth of field. | The distance above the slide in which the object is in good focus. |
| Eyepiece/Ocular lens. | Magnifies/Enlarges the image. (10x) |
| Objective lens. | Enlarges the image. (10x/40x) |
| Body tube. | Holds the lenses. |
| Nose piece. | Rotates to change objective lens from one power to another. |
| Stage. | Platform on which the specimen is mounted for observation. |
| Clips. | Hold the slide in place. |
| Diaphragm. | Controls the light quantity passing through the specimen. |
| Arm. | Holds the tube and stage used for carrying the microscope. |
| Base. | Supports the microscope, used for carrying it. |
| Coarse adjustment knob. | Used for focusing of the low power objective. |
| Fine adjustment knob. | Used for final focusing of the low power objective and all focusing of the high power objective. |
| Light microscope. | A microscope that uses light to produce an enlarged view of an image. |
| Simple microscope. | Magnifying glass. (Single lens) |
| Compound microscope. | Two lenses, images are inverted and reversed. |
| Phase contrast microscope. | Allows the details within living specimens to be seen without staining. |
| Electron microscopes. | Can not observe living or thick specimens, uses beams of electrons to magnify images, used to view smallest cellular organelles. |
| Dissecting microscope. | Examines objects and small organisms, images appear in 3D, two eyepieces. |
| Ultracentrifuge. | Separates the parts of cells according to their different densities. |