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Microscope
Biology
Question | Answer |
---|---|
How many microscopes should you lift at once? | One at a time. |
How should you hold a microscope? | One hand on the arm and the other on the base. |
Do not use your ____________ to clean the lenses, instead use _________ ___________. | Fingers, lens paper. |
Always start on ____________ power. | Low. |
Use the ___________ adjustment knob when on low power, then the _________ adjustment for better resolution. | Coarse, fine. |
Why should you look at the microscope from the side when switching to high power? | To make sure the objective doesn't hit the slide. |
True or False: Only use the coarse adjustment knob on high power. | False, fine adjustment knob. |
Wet Mount Slide: 1. Place a ________ of water on the slide. 2. Place the __________ in the water. 3. Lower the cover slip on a -- degree angle to avoid ________ __________. | Drop, specimen, 45, air bubbles. |
Magnification. | How an image is enlarged under the microscope. |
Low power has ___________ magnification. | Lower. |
High power has _____________ magnification. | Higher. |
Total magnification formula. | Objective x eyepiece. |
Resolution. | Amount of detail you can see of an image. |
Field of view. | The diameter of light you see when looking through a microscope. |
As magnification increases, the field of view ____________. | Decreases. |
Low power has a _________ field of view. | Larger. |
High power has a _____________ field of view. | Smaller. |
True or False: Low power is darker. | False, brighter. |
True or False: High power is darker. | True. |
Depth of field. | The distance above the slide in which the object is in good focus. |
Eyepiece/Ocular lens. | Magnifies/Enlarges the image. (10x) |
Objective lens. | Enlarges the image. (10x/40x) |
Body tube. | Holds the lenses. |
Nose piece. | Rotates to change objective lens from one power to another. |
Stage. | Platform on which the specimen is mounted for observation. |
Clips. | Hold the slide in place. |
Diaphragm. | Controls the light quantity passing through the specimen. |
Arm. | Holds the tube and stage used for carrying the microscope. |
Base. | Supports the microscope, used for carrying it. |
Coarse adjustment knob. | Used for focusing of the low power objective. |
Fine adjustment knob. | Used for final focusing of the low power objective and all focusing of the high power objective. |
Light microscope. | A microscope that uses light to produce an enlarged view of an image. |
Simple microscope. | Magnifying glass. (Single lens) |
Compound microscope. | Two lenses, images are inverted and reversed. |
Phase contrast microscope. | Allows the details within living specimens to be seen without staining. |
Electron microscopes. | Can not observe living or thick specimens, uses beams of electrons to magnify images, used to view smallest cellular organelles. |
Dissecting microscope. | Examines objects and small organisms, images appear in 3D, two eyepieces. |
Ultracentrifuge. | Separates the parts of cells according to their different densities. |