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How many microscopes should you lift at once? One at a time.
How should you hold a microscope? One hand on the arm and the other on the base.
Do not use your ____________ to clean the lenses, instead use _________ ___________. Fingers, lens paper.
Always start on ____________ power. Low.
Use the ___________ adjustment knob when on low power, then the _________ adjustment for better resolution. Coarse, fine.
Why should you look at the microscope from the side when switching to high power? To make sure the objective doesn't hit the slide.
True or False: Only use the coarse adjustment knob on high power. False, fine adjustment knob.
Wet Mount Slide: 1. Place a ________ of water on the slide. 2. Place the __________ in the water. 3. Lower the cover slip on a -- degree angle to avoid ________ __________. Drop, specimen, 45, air bubbles.
Magnification. How an image is enlarged under the microscope.
Low power has ___________ magnification. Lower.
High power has _____________ magnification. Higher.
Total magnification formula. Objective x eyepiece.
Resolution. Amount of detail you can see of an image.
Field of view. The diameter of light you see when looking through a microscope.
As magnification increases, the field of view ____________. Decreases.
Low power has a _________ field of view. Larger.
High power has a _____________ field of view. Smaller.
True or False: Low power is darker. False, brighter.
True or False: High power is darker. True.
Depth of field. The distance above the slide in which the object is in good focus.
Eyepiece/Ocular lens. Magnifies/Enlarges the image. (10x)
Objective lens. Enlarges the image. (10x/40x)
Body tube. Holds the lenses.
Nose piece. Rotates to change objective lens from one power to another.
Stage. Platform on which the specimen is mounted for observation.
Clips. Hold the slide in place.
Diaphragm. Controls the light quantity passing through the specimen.
Arm. Holds the tube and stage used for carrying the microscope.
Base. Supports the microscope, used for carrying it.
Coarse adjustment knob. Used for focusing of the low power objective.
Fine adjustment knob. Used for final focusing of the low power objective and all focusing of the high power objective.
Light microscope. A microscope that uses light to produce an enlarged view of an image.
Simple microscope. Magnifying glass. (Single lens)
Compound microscope. Two lenses, images are inverted and reversed.
Phase contrast microscope. Allows the details within living specimens to be seen without staining.
Electron microscopes. Can not observe living or thick specimens, uses beams of electrons to magnify images, used to view smallest cellular organelles.
Dissecting microscope. Examines objects and small organisms, images appear in 3D, two eyepieces.
Ultracentrifuge. Separates the parts of cells according to their different densities.
Created by: emarciante9