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BI Lab 4
| Term | Definition |
|---|---|
| all organisms are composed of one or more cells, the cell is the basic living unit of organization, and all cells arise from pre-existing cells | cell theory |
| compound light microscope | contains two or more lenses that collect and focus light passing through a transparent specimen producing a magnified image |
| magnification | the degree to which the image of a specimen is enlarged |
| resolution | resolving power; how well specimen detail is preserved during the magnifying process |
| contrast | the ability to see specimen detail against its background |
| field of view | the circle of light one sees in the microscope when looking through the eyepiece |
| decreases, meaning a smaller area of the slide is visible | as magnification increases, the field of view _______________ |
| working distance | the space between the slide and the objective |
| depth of field | vertical depth of an image that remains in sharp focus under a particular magnification |
| ocular or eyepiece | located in the upper end of the body tube. the magnification power is 10x |
| arm | functions as the handle of the microscope |
| objective lenses | attached to the revolving nosepiece. housed in one end of several steel tubes that are threaded into the revolving nosepiece |
| stage | large platform just below the revolving nosepiece |
| mechanical stage | apparatus located on the stage that holds a specimen mounted on a glass slide in place suspended over the stage opening |
| mechanical stage knobs | one moves the slide forward and back and the other moves the slide from side to side |
| coarse adjustment knob | used to move the stage up and down, so that you can bring the specimen into focus (only when using 4x) |
| fine adjustment knob | use when looking at objects using the low power objective (10x) and the high dry power objective (40x). used to bring the specimen into sharp focus |
| condenser | located between the diaphragm and the stage; converges light rays from the light source so that they pass through the specimen on the slide and into the objective lens |
| condenser adjustment knob | located underneath the stage on the left side just behind the condenser; adjusts the condenser for proper positioning to converge light rays passing through the specimen |
| iris diaphragm | lever located below the stage that regulates the amount of light passing through a specimen, thus improving the contrast of the image |
| illuminator | located below the condenser; contains the light source - a light bulb |
| base | lowermost part of the microscope and is in contact with the table |
| light switch | back of the microscope next to the electric cord mount |
| rheostat | varies the intensity of light coming from the illuminator; located on the top of the base |
| total magnification | multiply the power of the eyepiece times the power of the objective lens being used |
| permanent slide | purchased from a biological supply house and is sealed with a permanent glue |
| temporary wet mount | prepared in the lab usually using water as the suspension medium |
| parfocal | the ability of the microscope to remain in focus when switching from one objective lens to the next highest power |
| chloroplasts | function in photosynthesis and are necessary for plant life |
| cell wall | important for structural support and is not found in animal cells |
| plasma membrane | located inside the cell wall, is not visible bc it is pressed tightly against the cell wall and bc it is beyond the resolving power of the light microscope |
| cytoplasmic streaming | positions the chloroplasts toward light and distributes heat throughout the cell |
| central vacuole | provide hydrostatic pressure or turgor within individual cells |
| turgor | hydrostatic pressure |
| nucleus | control center of the cell; houses the DNA |
| plasma membrane | surrounds the cell and regulates what moves into and out of the cell |
| cytoplasm | the jelly-like material found between the cell's nucleus and plasma membrane |
| organelles | structures which perform specific cell functions |
| stereomicroscope | has two eyepieces that give a three-dimensional view of the specimen |
Created by:
pace_sauce
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