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BI Lab 4

all organisms are composed of one or more cells, the cell is the basic living unit of organization, and all cells arise from pre-existing cells cell theory
compound light microscope contains two or more lenses that collect and focus light passing through a transparent specimen producing a magnified image
magnification the degree to which the image of a specimen is enlarged
resolution resolving power; how well specimen detail is preserved during the magnifying process
contrast the ability to see specimen detail against its background
field of view the circle of light one sees in the microscope when looking through the eyepiece
decreases, meaning a smaller area of the slide is visible as magnification increases, the field of view _______________
working distance the space between the slide and the objective
depth of field vertical depth of an image that remains in sharp focus under a particular magnification
ocular or eyepiece located in the upper end of the body tube. the magnification power is 10x
arm functions as the handle of the microscope
objective lenses attached to the revolving nosepiece. housed in one end of several steel tubes that are threaded into the revolving nosepiece
stage large platform just below the revolving nosepiece
mechanical stage apparatus located on the stage that holds a specimen mounted on a glass slide in place suspended over the stage opening
mechanical stage knobs one moves the slide forward and back and the other moves the slide from side to side
coarse adjustment knob used to move the stage up and down, so that you can bring the specimen into focus (only when using 4x)
fine adjustment knob use when looking at objects using the low power objective (10x) and the high dry power objective (40x). used to bring the specimen into sharp focus
condenser located between the diaphragm and the stage; converges light rays from the light source so that they pass through the specimen on the slide and into the objective lens
condenser adjustment knob located underneath the stage on the left side just behind the condenser; adjusts the condenser for proper positioning to converge light rays passing through the specimen
iris diaphragm lever located below the stage that regulates the amount of light passing through a specimen, thus improving the contrast of the image
illuminator located below the condenser; contains the light source - a light bulb
base lowermost part of the microscope and is in contact with the table
light switch back of the microscope next to the electric cord mount
rheostat varies the intensity of light coming from the illuminator; located on the top of the base
total magnification multiply the power of the eyepiece times the power of the objective lens being used
permanent slide purchased from a biological supply house and is sealed with a permanent glue
temporary wet mount prepared in the lab usually using water as the suspension medium
parfocal the ability of the microscope to remain in focus when switching from one objective lens to the next highest power
chloroplasts function in photosynthesis and are necessary for plant life
cell wall important for structural support and is not found in animal cells
plasma membrane located inside the cell wall, is not visible bc it is pressed tightly against the cell wall and bc it is beyond the resolving power of the light microscope
cytoplasmic streaming positions the chloroplasts toward light and distributes heat throughout the cell
central vacuole provide hydrostatic pressure or turgor within individual cells
turgor hydrostatic pressure
nucleus control center of the cell; houses the DNA
plasma membrane surrounds the cell and regulates what moves into and out of the cell
cytoplasm the jelly-like material found between the cell's nucleus and plasma membrane
organelles structures which perform specific cell functions
stereomicroscope has two eyepieces that give a three-dimensional view of the specimen
Created by: pace_sauce