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Types of Microscopes
Question | Answer |
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Uses a combination of lenses magnifying the images of small objects | Optical light microscope |
Example of an optical light micoscope | Compound microscope |
The image viewed is against a bright lighted microscope field | Bright field |
The bright image of the specimen is visible against a dark field of view | Dark field |
Microscope that can be used to view bacteria and transparent specimens and uses a negative field | Dark field |
Enhance the density difference in the organelles causing certain structures to appear brighter/ darker than the others | Phase contrast |
Same with the phase contrast but it's effect is attained through technical optical methods w/c enhance density differences among organelles | Differential-inference-contrast |
Another technique for contrasting transparent cells where a certain type of protein can be determined | Immuno-fluorescent |
Introduced as a major development in illumination because it uses laser beams and special optics for optical sectioning of the specimen. A computer combines these optical section into 3D image projected on a screen. | Confocal |
Has a digital CCD camera attached to it and connected to a LCD or a computer monitor. Usually has no eyepieces to view objects directly. | Digital Microscope/ Microscope camera |
A Type of digital microscope which has the possibility mounting the camera, that would be an USB microscope | Trinocular type |
Uses two objectives and two eyepieces which makes it possible to view a specimen under angles to the human eye forming a stereo 3D optical version | Stereoscopic/ dissecting microscope |
The most advanced and powerful tool w/ the highest magnifying capacity. | Electron microscope |
Uses electrons to illuminate the tiniest particles | Electron microscope |
-Magnifies sample up to one million times - It looks at a sample all at once in 2D form | Transmission Electron Microscope (TEM) |
- Magnifies a sample up to 100 000X - Scans the surface bit by bit and produce 3D images of the surface of objects | Scanning electron microscope (SEM) |
Combines elements of SEM and TEM and can resolve single atoms in a sample. It gives 3D images of objects down to the atomic level. | Scanning tunneling Electron Microscope (STEM) |