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Bio Microscopes

Compound Light Microscopes 1. Brightfield (used in high school) 2. Phase Contrast 3. Nomarski 4. Flourescence 5. Confocal 6. Dissecting microscope
Phase Contrast Alignment of light beam that lets us see organelles >> highly refractive structure
Brightfield 1. 2 lenses: Eyepiece (10x) 2. 3 Objectives: Scanning (4x), Low (10x), High (40x) **Image appearance: Inversion, reversal
Resolving Power The higher the resolving power, the more detail (but less objects in field of view)
Field of View Diameter of view in which specimens are observed. Low: 1.5 mm or 1500 micrometers High: 0.375mm or 375 micrometers
TM Total magnification
Normarski Differential interference suited for viewing LIVE specimens
Fluorescence Certain cellular structures tagged (dyed) w/fluorescent molecules **Colored!
Confocal Lasers as light source
Dissecting Microscope 1. External structures of plants and animals 2. Aka stereomicroscope 3. 3D image 4. No reversal or inversion 5. Bifocal 6. 1 objective (50x) 7. Range of TM: 6-50x
Electron Microscope 1. View detailed structures of cell-liked organelles 2. Magnification 250,000x 3. Beam of electrons instead of light 4. Non-living!
Scanning Electron Microscope (SEM) Beam of electrons back and forth across the surface
Scanning Tunneling Microscope 1. Views DNA, which repairs enzympes 2. Atoms of NaCl crystal
Transmission Electron Microscope 1. Cross-section 2. Beam of electrons thru THIN specimen (0.1 micro) 3. 3D 4. Black and white
Created by: jubie