click below
click below
Normal Size Small Size show me how
Bio Microscopes
Term | Definition |
---|---|
Compound Light Microscopes | 1. Brightfield (used in high school) 2. Phase Contrast 3. Nomarski 4. Flourescence 5. Confocal 6. Dissecting microscope |
Phase Contrast | Alignment of light beam that lets us see organelles >> highly refractive structure |
Brightfield | 1. 2 lenses: Eyepiece (10x) 2. 3 Objectives: Scanning (4x), Low (10x), High (40x) **Image appearance: Inversion, reversal |
Resolving Power | The higher the resolving power, the more detail (but less objects in field of view) |
Field of View | Diameter of view in which specimens are observed. Low: 1.5 mm or 1500 micrometers High: 0.375mm or 375 micrometers |
TM | Total magnification |
Normarski | Differential interference suited for viewing LIVE specimens |
Fluorescence | Certain cellular structures tagged (dyed) w/fluorescent molecules **Colored! |
Confocal | Lasers as light source |
Dissecting Microscope | 1. External structures of plants and animals 2. Aka stereomicroscope 3. 3D image 4. No reversal or inversion 5. Bifocal 6. 1 objective (50x) 7. Range of TM: 6-50x |
Electron Microscope | 1. View detailed structures of cell-liked organelles 2. Magnification 250,000x 3. Beam of electrons instead of light 4. Non-living! |
Scanning Electron Microscope (SEM) | Beam of electrons back and forth across the surface |
Scanning Tunneling Microscope | 1. Views DNA, which repairs enzympes 2. Atoms of NaCl crystal |
Transmission Electron Microscope | 1. Cross-section 2. Beam of electrons thru THIN specimen (0.1 micro) 3. 3D 4. Black and white |