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Nat 5 Cells

Nat 5 Cell culture/DNA

what is an aseptic technique? technique designed to minimize growth of unwanted microorganisms
what are the precautions you take to minimize the growth of unwanted microbes? wear a lab coat, work close to a Bunsen burner, limit time the agar plates are open, disinfect surfaces and equipment.
what does every cell culture need? Cell culture requires an appropriate liquid or solid medium such as nutrient agars (solids) or broths (liquid) in which the cell can grow and divide.
How are microorganisms grown on an industrial scale? Micro organisms are grown in industry on a large scale in container called a fermenter.
What factors need to be controlled in cell culture? In cell culture factors such as temperature, pH and oxygen levels are controlled to ensure optimal growth of cells.
How do you disinfect the fermenter after use? Sterilise the fermenter by heating fermenter to 120˚C at high pressure for 20 minutes.
Where is the genetic information kept in the cell and how is it organised? The nucleus of living cells contain genetic information organised into chromosomes
What are chromosomes made up of? Chromosomes are made up DNA divided into regions called genes.
What substance are genes made up of? DNA is a complex substance that forms the genes of all living organisms.
What does DNA code for? DNA carries the genetic information coding for proteins.
What is the shape of DNA? A single DNA molecule is coiled into a double stranded helix.
What are three parts a DNA nucleotide is made up of? DNA nucleotides are made up of 3 parts; deoxyribose sugar, phosphate and bases.
What are the four types of DNA bases? There are four different types of bases known as Adenine (A), Thymine (T), Guanine (G) and Cytosine (C).
What keeps the two strands of DNA together? The strands of the DNA helix are held by bonds between complementary bases on each strand.
How do DNA bases pair with each other? Base A only pairs with T and base G only pairs with C.
What does the sequence of bases code for? The sequence of bases A, T G and C make up the genetic code for proteins by coding for amino acids.
Created by: kyle_academy



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