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DNA replication

prokaryotic DNA replication

QuestionAnswer
What is the point where the two parental DNA strands separate to allow replication? replication fork
What is the multi-protein machine that mediateds bacterial DNA replication? replisome
What is the unique site on chromosomal DNA where replcation begins? origin of replication (ORI)
What is the ORI of E. coli ? oriC
What is the enzyme that unwinds a DNA or RNA double helix? helicase
What is the fragment of E. coli DNA polymerase I that lacks the 5' to 3' exonuclease activity of the parent enzyme? Klenow fragment
What is the strand that is made discontinuously in DNA replication? lagging strand
What is the strand that is made of continuously in DNA replication? leading strand
What is the enzyme that facilitates the joining of DNA strands together by catalyzing the formation of a phosphodiester bond? ligase
What is the enzyme that catalyzes the formation of an RNA primer during DNA replication? primase
What is the enzyme that plays a role in primer removal and gap filling between Okazaki fragments in E. coli DNA replicaton? DNA polymerase I
What is the enzme that catalyzes DNA strand elongation in E.coli replication? DNA polymerase III
What is the protein that binds to single-stranded DNA and keeps it from base pairing with complemetary strand during DNA replication? SSB
What is the protein that helps tether DNA polymerase to the DNA during replication to allow highly processive synthesis? sliding clamp
What are the small DNA fragments created by discontinuous synthesis of the lagging strand? Okazaki fragments
What is the enzyme that introduces negative supercoil into DNA during E. coli DNA replication? gyrase
Created by: mingjiuanwu