Pathogen should be found in all sick individuals, but not in any healthy individuals.

Pathogen must be isolated and grown in culture.

Pathogen must be inoculated into healthy organism AND cause disease.

Pathogen must be isolated from once-healthy organism, grown in culture, AND found to be identical to the original pathogen.

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Koch's Postulates

Term	Definition
Koch's 1st Postulate	Pathogen should be found in all sick individuals, but not in any healthy individuals.
Koch's 2nd Postulate	Pathogen must be isolated and grown in culture.
Koch's 3rd Postulate	Pathogen must be inoculated into healthy organism AND cause disease.
Koch's 4th Postulate	Pathogen must be isolated from once-healthy organism, grown in culture, AND found to be identical to the original pathogen.