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National 5 Cell Bio
Revision for DNA/proteins/GE
| Question | Answer |
|---|---|
| The order of bases in DNA determines order of... | amino acids |
| enzymes are made of | protein |
| What is the function of hormones | chemical messengers |
| what protein defends the body against disease | antibodies |
| what protein speeds up chemical reactions | enzymes |
| a ring of genetic material in a bacteria is a | plasmid |
| enzymes are also called this | biological catalysts |
| breaks down H2O2 to oxygen and water | catalase |
| breaks down protein to amino acids | pepsin |
| breaks down starch to maltose | amylase |
| breaks down fats to fatty acid and glycerol | lipase |
| DNA and mRNA are made of | nucleotides |
| enzyme reactions work best at the | optimum |
| where an enzyme binds with a substrate | active site |
| this happens to enzymes at high temperatures | denatures |
| enzyme involved In a synthesis reaction | phosphorylase |
| enzymes only work on one substrate, they are | specific |
| carries genetic code from nucleus to the ribosome | mRNA |
| where protein synthesis occurs | Ribosome |
| base partner of A | T |
| base partner of T | A |
| base partner of G | C |
| base partner of C | G |
| where AEROBIC respiration occurs | mitochondria |
| process to transfer human genes to bacteria | genetic engineering |
| modified plasmids are placed here | a different bacteria cell |
| human hormone produced by genetic engineering | insulin |
| genetic information can pass between bacteria this way | horizontal gene transfer i.e. plasmids transferred between bacteria |
| What is the enzyme that breaks down fat | lipase |
| What is the enzyme that breaks down protein | pepsin |
| What is the enzyme that breaks down starch | amylase |
| What is the enzyme that breaks down hydrogen peroxide | catalase |
| what is the enzyme that makes starch | phosphorylase |
| what is the enzyme that makes amino acids | pepsin |
| What does catalase reacting with hydrogen peroxide produce | water and oxygen |
| what does amylase reacting with starch produce | maltose |
| Name a degrading enzyme | catalase OR amylase OR pepsin OR lipase |
| what is the optimum pH for catalase | 9 (alkali) |
| What is the optimum pH for pepsin | 3 (acid) |
| What two products does lipase reacting with fat produce | fatty acids and glycerol |
| what are the three parts to a DNA nucleotide | sugar, phosphate and base |
| What is the 3D structure of DNA called | double helix |
| What does DNA code for | protein |
| Where is DNA found | nucleus |
| What is the journey of a mRNA molecule when it carries the genetic code | nucleus to ribosome |
| What two effects does the order of amino acids cause | the structure and therefore the function of the protein |
| Name 4 types of proteins | antibodies, enzymes, receptors, hormones and structural proteins |
| what is the function of structural proteins | to provide support in membrane |
| what is the function of receptors | to bind with a specific hormone at the target tissue |
| what structures on chromosomes code for a specific protein | genes |
| what is meant by complementary bases | A and T, C and G |
| Name a differences between DNA and RNA | DNA has 2 strands and RNA is only 1 strand |
| What is meant by denaturing | change the shape of the active site |
| Name two factors that affect enzymes | pH and temperature |
| what molecule cuts genes out of chromosomes in genetic engineering | enzymes |
| What is the first stage of genetic engineering | identify section of DNA that contains required gene from chromosome |
| What is the second stage of genetic engineering after identifying gene on chromosome | extract required gene with enzymes |
| What is the third stage of genetic engineering after extracting gene from chromosome | extract plasmid from bacterial cell and cut open with enzymes |
| What is the fourth stage of genetic engineering after extracting plasmid for bacterial cell | insert human gene into plasmid |
| What is the fifth stage of genetic engineering after | insert plasmid into different bacteria |
| What is the sixth stage of genetic engineering after inserting plasmid into different bacteria | grow bacteria in fermenter and extract protein produced |
| Give an advantage of genetic engineering | produce large quantities of protein OR fast process |
| Give an example of a genetically engineered protein | insulin OR human growth hormone |