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BZ 310- Lab Final
Question | Answer |
---|---|
three levels of cell cultures | culture, subculture, cell line |
cell counting | dilution x 1000 x (cells counted)/(mL) |
.025%= | .25mg/mL |
freezing medium contains what and why? | DMSO to prevent freezing damage |
growth medium | Grace's medium, 10% bovine serum |
three growth phases | lag, log, plateau |
three things used to prevent contamination | laminar flow cabinets, 70% ethanol, UV lights |
most common problem for cell culture | mycoplasma from the mouth (breath) |
flow cabinets cannot be used for | pathogens since they flow outward |
Lowry | one part detects tryptophan and tyrosine, other part detects peptide bonds |
Bradford | coomassie brilliant blue color change proportional to the concentration of protein |
Folin-Ciocalteau | (Lowry) detects trp/tyr by phosphomolybdate salts by reacting with them |
Biuret reaction | (Lowry) turns purple when copper sulfate reacts with peptide bonds |