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BZ 310- Lab Final

three levels of cell cultures culture, subculture, cell line
cell counting dilution x 1000 x (cells counted)/(mL)
.025%= .25mg/mL
freezing medium contains what and why? DMSO to prevent freezing damage
growth medium Grace's medium, 10% bovine serum
three growth phases lag, log, plateau
three things used to prevent contamination laminar flow cabinets, 70% ethanol, UV lights
most common problem for cell culture mycoplasma from the mouth (breath)
flow cabinets cannot be used for pathogens since they flow outward
Lowry one part detects tryptophan and tyrosine, other part detects peptide bonds
Bradford coomassie brilliant blue color change proportional to the concentration of protein
Folin-Ciocalteau (Lowry) detects trp/tyr by phosphomolybdate salts by reacting with them
Biuret reaction (Lowry) turns purple when copper sulfate reacts with peptide bonds
Created by: melaniebeale