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BZ 310- Lab Quiz 3

QuestionAnswer
partition coefficient lower= will pass through the membrane (lipid soluble)
chloroform-methanol lipid solvent
Triton X-100 nonionic detergent
Sodium dodecyl sulfate ionic detergent
most water soluble compound in list n-propanol
least water soluble compound in list glycerol
5 properties that make E. coli DH5-alpha safe pro/thi auxotroph, no polysaccharide layer, cell wall defects, lack of enzyme that degrades plasmid DNA, lack of ability to recombine DNA
EDTA destabilizes cell wall and protects DNA from degradation
restriction enzymes depend on (2) Mg2+ ions and neutral pH
find ug DNA absorbance at 260 (DNA) x 50 ug/mL x 15 dilution x 1mL/1000 uL x 50 uL
absorbance is directly proportional to concentration of the sample
hypotonic less solutes outside of cell, cell bursts
bacterial trait helpful to molecular biologist but not doctor altered so it cannot make one sick
the two different types of DNA present in bacteria are similar in what sense? double stranded, encode for genes
wavelength DNA 260nm
wavelength protein 280nm
how did we differentiate between transformed and non-transformed cells? whether or not they grew on antibiotics
two types of competent cells yeast (naturally), any cell treated w/ CaCl2
crenate hypertonic
calculate CFU/mL colonies/mL/(1/dilution)
Created by: melaniebeale