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Enzymes for MMBIO

Type II restriction endonuclease cleaves DNA at specific base sequences
DNA ligase Joins two DNA molecules or fragments
DNA polymerase I (E. coli) Fills single-stranded gaps in duplex DNA by stepwise addition of nucleotieds to 3' ends
Reverse transcriptase Makes a DNA cop of an RNA molecule
Polynucleotide kinase Adds a phosphate to the 5'-OH end of a polynucleotide, to label it or permit ligation
Terminal transferase Adds homopolymer tails to the 3'-OH ends of a linear duplex
Exonuclease III Removes nucleotide residues from the 3' ends of a DNA strand
Bacteriophage (beta) exonuclease Removes nucleotides from the 5' ends of a duplex to expose single-stranded 3' ends
Alkaline phosphatase Removes terminal phosphates from the 5' end, the 3' end, or both
Created by: ksheehy96