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Enzymes for MMBIO
Term | Definition |
---|---|
Type II restriction endonuclease | cleaves DNA at specific base sequences |
DNA ligase | Joins two DNA molecules or fragments |
DNA polymerase I (E. coli) | Fills single-stranded gaps in duplex DNA by stepwise addition of nucleotieds to 3' ends |
Reverse transcriptase | Makes a DNA cop of an RNA molecule |
Polynucleotide kinase | Adds a phosphate to the 5'-OH end of a polynucleotide, to label it or permit ligation |
Terminal transferase | Adds homopolymer tails to the 3'-OH ends of a linear duplex |
Exonuclease III | Removes nucleotide residues from the 3' ends of a DNA strand |
Bacteriophage (beta) exonuclease | Removes nucleotides from the 5' ends of a duplex to expose single-stranded 3' ends |
Alkaline phosphatase | Removes terminal phosphates from the 5' end, the 3' end, or both |