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Bio 272

QuestionAnswer
Organ culture Tissue left at gas-liquid interface. Histological structure is maintained
Explant culture Tissue is left at solid-liquid interface and migrates to form an outgrowth
Dissosociated cell culture Disaggregated tissue that forms a monolayer at solid-liquid interface
Organotypic culture Different cells are co-cultured with or without a matrix forming an organotypic structure
Disagregation Use EDTA and trypsin. Or manual
Trypan blue Test for if cells living. Non-dead cells do not stain, while dead cells stain blue. Cell membrane permeable. Vital dyes
Metabolic assay substrates MTT and resazurin
What is prestoBlue Example of a resazurin based dye
Dna binding dyes DAPI, propidium iodide
Propidium iodide Intercalating agent requires a permeabilised cell
Longpass emission filter 580nm
Alexa Fluor green fluorescent dye bound ot agglutining
Phenol red pH indicator. Normal conditions at (7.35). Bacterial contamination acidified the sample.
Medium constituents inorganic salts, amino acids, sugars, ions, trace elements, vitamin, cofactores and ions and glucose
Advantages of Serum Binds and neutralises toxins, complete set of additional growth factors, attachment and spreading factors, binding and transpot proteins; protease inhibitors; buffering capacity; provides trace elements
Disadvantages of Serum Not chemiclly defined; composition varies a lot; source of contamination; increases dificuties and cost of down stream processing; expensive component of the culture medium
Created by: 1storbroke
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