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MM and Enzymes
For Honors Bio Test
Term/Question | Definition/Answer |
---|---|
Why is carbon a building block? | Because it can bond with 4 other molecules |
4 types of macro-molecules | Lipids, Proteins, Carbohydrates, and Nucleic Acids |
examples of a carbohydrate | glucose, cellulose, and starch |
examples of a lipid | fats, waxes, oils, and cutin |
examples of a protein | enzymes, hormones, antibodies, and callegen |
examples of nucleic acid | DNA & RNA |
monomer of a carbohydrate | saccharide |
monomer of a lipid | phospholipid |
monomer of a protein | amino acid |
monomer of nucleic acid | nucleotide |
function of a carbohydrate | "easy energy" |
function of a lipid | long term energy |
function of a protein | "build and bind" |
function of nucleic acid | produce protein |
A phospholipid is made of... | glycerol and fatty acids |
A nucleotide is made of... | phosphate, sugar, and a base |
Amino acids strands are made of... | amino acid and pepto bonds |
Procedure for testing for carbohydrates | STARCH- fill test tubes with 5 ml of substrate add 5 drops of iodine record color change SUGAR- fill test tubes with 5 ml of substrate add 10 drops of Benedict place in heated water record color change |
Chemical used in testing for carbohydrates | Iodine (starch) and Benedict (sugar) |
Color of positive reaction (carbohydrates) | Iodine- blue-black Benedict- orange-red |
Example of that would test positive for carbohydrates | Iodine- potato Benedict- honey, lettuce, and juice |
Procedure for testing for lipids | divide a brown paper bag into 10 sections place a sample of each food item in each section place aside and wait for the water to dry positive=grease stain |
how do you know its a positive reaction ?(lipids) | grease spot |
Example of that would test positive for lipids | corn oil and butter |
Procedure for testing for proteins | Add biuret to the substance being tested and look for a color change |
Chemical used in testing for proteins | Biuret |
Color of positive reaction (proteins) | purple |
Example of that would test positive for proteins | yogurt, gelatin, and butter |
What is "lock and key"? | It describes how there is only on enzyme per substrate much like how there is only one lock for each key. |
What is the active sight? | The place where the enzyme and the substrate bind. |
What is activation energy? | the energy needed to start a reaction |
What does it mean to "denature" an enzyme? | To warp the active site so that the enzyme can no longer bind with its substrate and a reaction cannot take place |
What does pH and temperature do to an enzyme? | It denatures it. |
What enzyme is used in the liver lab? | catalase |
what substrate is used in the liver lab? | Hydrogen Peroxide (H2O2) |
What is the reactant in the liver lab? | Hyrdrogen Peroxide (H2O2) |
What is the product in the liver lab? | Water (H2O) ans Oxygen (H2) |
What is the equation for the liver lab? | H2O2 ---> H2O + H2 enzyme: catalase |
What is the procedure for the liver lab? | -pour 2ml of water in tube 1 and 2ml of hydrogen peroxide(H2O2) in the remaing 6 tubes -add the liver/potato/sand -measure the reaction by measuring from the bottom of the tube to the top of the bubbles |
Independent variable in liver lab? | food items |
Dependent variable in liver lab? | reaction height |
controlled variable in liver lab? | amount of Hydrogen Peroxide (H2O2) |