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Stack #160523
Microbiology Test #2 Review Dr. Bharathi
| Question | Answer |
|---|---|
| What is an organism that produces superoxide dimutase and catalase? | Aerobe |
| What is not a disadvantage for a direct microscope count? | Sample volume of an unknown. |
| What are the disadvantages of direct microscope count? | Motile bacteria are difficult to count, dead cells are likly to be counted, high concentrations of cells are requred to be countable. |
| What is the advantage of direct microscope count? | No incubation time is required. |
| What do hydrothermal vents on the ocean floor useas a carbon source? | CO2 |
| What is in nutrient agar? | peptone & beef extract |
| What do producers in the vents use? | sulfides |
| What is the direct method to count microbial growth? | metabolic activity |
| What is the best pH for most bacteria? | 7 |
| What is the best temperature for optimum growth of mesophiles? | 25 |
| What is the best temperature for optimum growth of psychrophiles? | 15 |
| What is the best temperature for optimum growth of psychotrophs? | 20-30 |
| What catalyzes 2H202---into 2H2O + O2? | Catalase |
| What is an organic growth factor? | NAD+ |
| What is not used to determine the metabolic growth factor? | Turbidity |
| What is an organism that uses oxygen or grows without oxygen? | facultative anaerobe |
| What is not a disadvantage to determine viable cells? | viable plate count |
| How do salts and sugars preserve food? | creates a hypertonic environment |
| What makes H202 + 2H+ ----into 2H20? | peroxidase enzyme |
| What is a common intermediate between catabolic and anabolic pathways? | ATP |
| When are gram positive bacteria most susceptible to penicillin? | Log phase |
| What organisms are capable of respiration? | anaerobic |
| What is generated by the flow of protons across the cell membrane? | ATP |
| What neutralizes acids in the medium? | buffers |
| What measures microbial growth? | metaboic activity & glocose consumption |
| Inwhat mediums do gram positive organisms grow such as staphylococcus aureus colonies? | selective & differential |
| How do gram positive organisms grown in selective & differential mediums look? | yellow halo surrounds |
| What is a step in glycolysis? | oxidation |
| What do aeobic and anaerobic respiration have in common? | final receptor of electrons are different in both |
| What is the oxidation of glucose with organic molecules serving as electron receptors? | fermintation |
| What is not necessary for respiration? | oxygen |
| What is an example of cyanobacteria? | photoautotroph |
| What uses CO2 for carbon and H2 for energy? | chemoautotroph |
| What is involved in aerobic respiration and oxidized in the Krebs Cycle? | pyruvic acid |
| What happens to a bacterial culture grown in glucose? | peptide medium causes the pH to increase most likely using peptides |
| What does phosphoeno pyruvatie into pyruvic acid generate? | ATP |
| What is happening when ATP general when protons are moved across a membrane? | chemiosmotic mechanism |
| What does photophosphrylation require? | CO2 |
| What is not an enzyme? | Coenzyme A |
| What is not an end product of fermentation? | Pyruvic acid |
| What has bacteria chlorophylls and uses alcohols for carbon? | photoheterotrophs |
| Fermentation is defined as what? | Metabolic process |
| Fermentatin releases what? | energy from sugar |
| Fermentation does not require what? | oxygen |
| How does fermentation occur? | anaerobically |
| What is the oxidation of intrmediate metabolic compounds? | substrate level phosphorylation |
| What is metabolized in the Krebs Cycle or citric acid cycle? | fattyacids |
| What is the energy released as carrier molecules and are oxidized and used to generte ATP? | oxidative phoshorylation |
| What does not produce 3 ATPs? | pentose hophate pathway |
| What uses glycose for carbon and energy? | chemoheterotroph |
| How does a strictly fermentative bacteria produce energy? | glycolyis only |
| What is not an energy source for a photautotroph? | CO2 |