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Sel & Diff Media

EMB, PEA, MSA, Hektoen

QuestionAnswer
Mannitols Salt Agar (Type) Selective & Differential
Mannitols Salt Agar (Color) Pink
MSA, Selective & Differential for: staphylococci
MSA, Selective Agent Sodium Chloride
MSA, Differential Agent Mannitol fermentation
Mannitol fermentation is indicated by production of acid=color change from red to yellow
Oranism positive for mannitol fermentation Staphylococcus aureus
Oranism negative for mannitol fermentation Staphylococcus epidermidis
Eosin Ethylen Blue Agar (EMB) confirmation of a presumptive test for coliform in water milk
EMB is selective for: enteric organisms
Members of the Salmonells & Shigella group will develop on the media Eosin Ethylen Blue Agar
lactctosr fomenters (E.Coli) will show _____ on Eosin Ethylen Blue Agar dark centers w/ a green sheen
Eosin Ethylen Blue Agar (color) Maroon
Hektoen Enteric Agar Slective & differential for gram-neg enteric organisms
Salmonella will grow greenish blue w/ black center on Hektoen Enteric Agar
E. coli will grow _________ colonies on Hektoen Enteric Agar orange
Shigella colonies will grow _______ on Hektoen Enteric Agar greenish blue
Phenylethyl Alcohol Agar (PEA) undefined (complexed) media
Selects for gram-pos organisms Phenylethyl Alcohol Agar (PEA)
PEA is commonly used on ______ cultures mixed
PEA _________ the growth of most gram-neg organisms inhibits
PEA hibits by interfering with _________ DNA synthesis
PEA consists of nurtients, salt, 0.25% phenylethyl alcohol
A high concentration of phenylethyl alcohol is _________ toxic to most organisms
Phenylethyl Alcohol Agar (PEA)(color) straw colored
Hektoen Enteric Agar (color) green
Created by: Chicago1
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