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Sel & Diff Media
EMB, PEA, MSA, Hektoen
| Question | Answer |
|---|---|
| Mannitols Salt Agar (Type) | Selective & Differential |
| Mannitols Salt Agar (Color) | Pink |
| MSA, Selective & Differential for: | staphylococci |
| MSA, Selective Agent | Sodium Chloride |
| MSA, Differential Agent | Mannitol fermentation |
| Mannitol fermentation is indicated by | production of acid=color change from red to yellow |
| Oranism positive for mannitol fermentation | Staphylococcus aureus |
| Oranism negative for mannitol fermentation | Staphylococcus epidermidis |
| Eosin Ethylen Blue Agar (EMB) | confirmation of a presumptive test for coliform in water milk |
| EMB is selective for: | enteric organisms |
| Members of the Salmonells & Shigella group will develop on the media | Eosin Ethylen Blue Agar |
| lactctosr fomenters (E.Coli) will show _____ on Eosin Ethylen Blue Agar | dark centers w/ a green sheen |
| Eosin Ethylen Blue Agar (color) | Maroon |
| Hektoen Enteric Agar | Slective & differential for gram-neg enteric organisms |
| Salmonella will grow greenish blue w/ black center on | Hektoen Enteric Agar |
| E. coli will grow _________ colonies on Hektoen Enteric Agar | orange |
| Shigella colonies will grow _______ on Hektoen Enteric Agar | greenish blue |
| Phenylethyl Alcohol Agar (PEA) | undefined (complexed) media |
| Selects for gram-pos organisms | Phenylethyl Alcohol Agar (PEA) |
| PEA is commonly used on ______ cultures | mixed |
| PEA _________ the growth of most gram-neg organisms | inhibits |
| PEA hibits by interfering with _________ | DNA synthesis |
| PEA consists of | nurtients, salt, 0.25% phenylethyl alcohol |
| A high concentration of phenylethyl alcohol is _________ | toxic to most organisms |
| Phenylethyl Alcohol Agar (PEA)(color) | straw colored |
| Hektoen Enteric Agar (color) | green |