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Microbiology Tests
| Question | Answer |
|---|---|
| hydrolysis of starch medium | starch agar |
| hydrolysis of starch substrate | starch |
| hydrolysis of starch enzyme | amylase |
| hydrolysis of starch reagent | iodine |
| hydrolysis of starch product | maltose |
| hydrolyssis of starch positive | amber |
| hydrolysis of starch negative | black |
| hydrolysis of gelatin medium | gelatin |
| hydrolysis of gelatin substrate | gelatin |
| hydrolysis of gelatin enzyme | gelatinase |
| hydrolysis of gelatin reagent | none |
| hydrolysis of gelatin end product | amino acids |
| hydrolysis of gelatin positive | liquid |
| hydrolysis of gelatin negative | solid |
| fermentation of carbs medium | phenol broth + proteins |
| fermentation of carbs substrate | glucose, sucrose, lactose, mannitol |
| fermantation of carbs pathway | glycolysis |
| fermantation of carbs reagent | phenol red |
| fermentation of carbs end product | organic acid, atp, co2 |
| fermentation of carbs positive | yellow, acid/gas |
| fermentation of carbs negative | red/control |
| litmus fermentation substrate | lactose |
| litmus fermentation enzyme | fermentation |
| litmus fermentation reagent | co2 indicates glycolosis |
| litmus fermentation end product | organic acid,atp. co2 |
| litmus fermentation pos | pink with or without curd |
| limus fermentation neg | control |
| litmus alkaline substrate | caesin |
| litmus alkaline enzyme | protease |
| litmus alkaline end | small peptides and amonia |
| litmus alkaline pos | blue |
| litmus alkaline neg | control |
| litmus pept sub | caesin lactal bumin |
| litmus pept enzyme | protease |
| litmus pept pos | grape/apple juice curd on bottom |
| litmus pept neg | basic |
| litmus pept end | free amino acids |
| litmus reduc substrate | litmus |
| litmus reduction pathway | etp |
| litmus reduc end | leucolitmus atp |
| litmus reduc pos | white acidic milk and apple |
| litmus reduc neg | basic |
| litmus curd formation substrate | acidic or basic conditions |
| litmus curd enzyme | fermentation or peptiniziation |
| litmus curd end | curd |
| litmus curd pos | curd |
| limus curd neg | no curd |
| reduction of nitrates medium | nitrate broth |
| reduc of nitrates substrate | kno3 |
| reduc nitrates pathway | etp |
| reduc of nitrates reagent | dan, sulfanic acid |
| reduction of nitrates end | atp kno3 |
| reduction of nitrates pos | magenta |
| reduction of nitrates neg | control |
| detection of catalase medium | tsa plate |
| catalase substrate | h202 |
| catalase enzyme | catalayse |
| catalase reagent | h2o2 |
| catalase end | h2o o2 |
| catalase pos | bubbles |
| catalase neg | no bubbles |
| decarboxylase medium | decarboxylase broth |
| decarbox substrate | arginine, lysine, omithine |
| decarbox enzyme | deacarbox |
| decarbox reagent | brom cresol purple |
| decarbox end | amine co2 |
| decarbox pos | violet |
| decarbox neg | yellow |
| urea medium | urea broth |
| urea substrate | urea |
| urea enzyme | urease |
| urea reagent | phenol red |
| urea end | co2 nh3 |
| urea pos | pink |
| urea neg | yellow |
| SIM Smedium | SIM |
| SIM S substrate | cystesine, h2s+feso |
| SIM S enzyme | ETC |
| SIM S end | ETC |
| SIM S pos | h2sgas atp black |
| sIm substrate | tryptophan |
| sIm enzyme | kovacs |
| sIm end | indole pyruvic acid |
| sIm pos | red ring |
| siM pos | movement from stab line |
| Methyl Red medium | MR-VP |
| MRed substrate | glucose |
| mred enzyme | glycolyusis |
| mred reagent | methyl red |
| mred end | atp pyruvic |
| mred pos | red |
| mred neg | yellow |
| vogue medium | MR-VP |
| vogue substrate | glucose |
| voge enzyme | alternate ehnzyme pathways |
| vogue reagent | diplopithol koh creatine |
| vogue end | atp amc organic acid |
| vogue pos | redring |
| vogue neg | basic |
| nitrogen medium | ammonium phosphate broth |
| nitrogen substrate | nh4h2po4 |
| nitrogen reagent | brom cresol purple |
| nitrogen end | nh3 phosphoric acid |
| nitrogen pos | yellow |
| nitrogen neg | burgundy/purple |
| carbon medium | sodium citrate broth |
| carbon substrate | sodium sitrate |
| carbon enzyme | citrase |
| carbon end | acetate oxalacetate |
| carbon pos | tubid |
| carbon neg | clear |
| obligate aerobe | slant tall |
| obligate anaerobe | thioglyollate |
| facultative anaerobe | all |
| micro aerophile | tall stab line |
| MSA medium | manitol salt agar |
| msa substrate | mannitol |
| msa enzyme | glycolysis |
| msa reagent | phenol red |
| msa end | organic acid atp |
| msa pos | yellow |
| msa neg | red |
| coagulase medium | rabbit plasma |
| coagulase substrate | sodium sirate |
| coagulase enzyme | coagulase |
| coagulase end | clotted plasma |
| coagulase pos | clot |
| coagulase neg | liquid |
| alpha radiation | partial rbc incomplete green |
| beta radiation | hemoglobin complete clear |
| gamma radiation | plasma red agaqr |
| latex medium | latex beads |
| latex pos | clumps |
| latex neg | no clumps |
Created by:
Tejirof
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