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Proteins
Cell bio
| Term | Definition |
|---|---|
| miRNA | may inhibit translation or lead to mRNA degradation |
| TFIIH | helicase and kinase to phosphorylate CTD of RNA pol II in transcription initiation |
| TFIID | binds first in transcription initiation |
| TBP | tata binding protein in transcription initiation |
| CTD | carboxyl terminal domain |
| ORC | origin recognition complex; marks origin throughout cell cycle |
| Gel shift assay | identify DNA-binding proteins |
| DNA affinity chromatography | identify proteins that bind a specific DNA sequence |
| Chromatin immunoprecipitation | determine when proteins are bound to a specific sequence in vivo |
| phosphatase | acts on 5' end during capping |
| guanyltransferase | adds GMP in 5'-5' linkage |
| CBC | binds cap; cap binding complex |
| CstF | cleavage stimulation factor F in 3' polyadenylation |
| CPSF | cleavage and polyadenylation specificity factor in 3' polyadenylation |
| IRES | internal ribosome entry site |
| hsp60 | barrel structure with isolation chamber; ATPase cap; mechanism for refolding |
| hsp70 | binds hydrophobic patches; binds as peptide exits ribosome |
| Ubiquitin | 7kd protein involved in degradation |
| E1 | ubiquitin activating enzyme |
| E2 | ubiquitin conjugating enzyme |
| E3 | ubiquitin ligase |
| Cdc6 | replication; recruits MCM helicase to ORC |
| Telomerase | replication; usus an RNA template to extend the 3' ends of linear chromosomes |
| elF4E | translation; binds the 5' cap and with eIF4G interacts with the polyA tail of mRNAs to form a circle and protect the mRNA |
| Cdc6 | binds with Cdt1 around the ORC in yeast during replication |
| Mcm | helicase that forms the prereplicative complex in yeast |