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LQ's Science Paper

QuestionAnswer
Sucrose efflux mediated by sweet proteins is a key step for? phloem transport
Using sucrose sensors, we ID-ed ... a subfamily of SWEET sucrose efflux transporters, SWEET 11 and 12
SWEET 11 and SWEET12 are localized to.. they are in... Plasma membrane of phloem, vascular tissue, major and minor veins
Mutants of SWEET 11 and 12 are defective in... phloem loading
Describe the 2 step mechanism of SWEET mediated export ... From parenchyma cells feeding to H+ coupled import into SE/CC
An effective mechanism to limit the ability of photosynthetic carbon in leaf apoplasm to prevent pathogen infections could be... to restrict intercellular transport to interface of adjacent phloem cells
SWEET can transport sucrose across the plasmodesmata, what are they in Arabidopsis and rice? SWEET 10- 15 in Arabidopsis, SWEET 11 and 14 in Rice
Sucrose is exported by ___ and feeds into ____ SWEET, and SUC2
Why were human cell lines suitable to represent the functional expression system for heterologous sucrose transporters? What did they find? They lack endogenous sucrose transport activity, so adding SWEET 10 + 15 allowed the human cells to accumulate sucrose
There is a preference for sucrose over ____ but no transport of ____ glucose, maltose
If you inject sucrose into frog eggs, ... there is a time dependent efflux of sucrose
How similar are SWEET 11 and 12? What happens when you mutate them? DKO? They are 88% similar in amino acids. Lines with TDNA insertions don't show significant phenotype, but at higher light, double mutant lines were smaller relative to WT controls
Mature leaves of the double mutant had high levels of sucrose at the end of the light photo period and end of the dark photoperiod- impaired ability to export sucrose from leaves
when phloem loading was blocked or plants were treated with sucrose, leaves had higher levels of ____ hexose
reduced efflux of sugars from leaves is expected to lead to reduced translocation of photo assimilates to roots
Mutants germinated in light - sugar had _____, adding sugar ___ reduced root length, rescued
Created by: sunying12
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