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PCR

Polymerase Chain Reaction

What is an exon? A gene
What is an intron? The parts between the genes (non coding blocks of DNA)
What are short tandem repeats? Short DNA sequences that are repeated, found in the introns
What are DNA samples treated with? Restriction enzymes
What is the function of restriction enzymes? To cut DNA at specific base sequences
What are DNA primers? Short DNA sequences that are complementary to the DNA at the sides of the STR.
What does PCR stand for? Polymerase Chain Reaction
What are the ingredients for PCR? DNA polymerase, DNA primers (with fluorescent markers), nucleotides
What is the temperature of the first stage of PCR? 95 degrees Celsius
What happens at the first stage of PCR? The DNA separates into two strands
What is the temperature of the second stage of PCR? 55 degrees Celsius
What happens in the second stage of PCR? The primers (with fluorescent markers) attach to the start of the STR
What is the temperature at the third stage of PCR? 70 degrees Celsius
What happens at the third stage of PCR? DNA polymerases attach. Nucleotides are added, extending the DNA from the primer. The STR repeated sequence and DNA adjacent is replicated
Created by: sphclrk
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