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Vocab
ch 17
| Term | Definition |
|---|---|
| Transcription | turning DNA into RNA |
| RNA Polymerase | enzyme that builds the RNA polymer |
| Prokaryotic (RNA Polymerase) | RNA polymerase (only 1) |
| Eukaryotic (RNA Polymerase) | RNA pol I,II, III |
| The Promoter | sequence of DNA that is where transcription is initiated |
| The Terminator | sequence of DNA (in bacteria) that signals the end of transcription |
| The Transcription Unit | the DNA that lies between the Promoter and the Terminator |
| Step 1 of Transcription | Initiation |
| Prokaryotic (Step 1) | RNA polymerase recognized the promoter and binds to it |
| Eukaryotic (Step 1) | promoter contains TATA box (RNA pol II) |
| Step 2 of Transcription | Elongation -RNA pol adds nucleotides & unwinds strand -RNA transcript peals away from DNA after made & DNA retwists |
| Step 3 of Transcription | Termination |
| Proks (Step 3) | once the terminator is transcribed RNA pol falls off & releases the mRNA transcript |
| Euks (Step 3) | RNA pol continues until transcribes a signal called polyadenylation signal (AAUAA) |
| AUG | thymine & start codon |
| UAA, UAG, UGA | stop codons |
| anticodon | attached to tRNA, and looks for mRNA |
| Step 1 of tRNA | charging tRNA -putting appropriate amino acid on the tRNA |
| Step 2 of tRNA | initiation of translation (look at figure in notes) |
| Step 3 of tRNA | Elongation (4 steps in notes) |
| Step 4 of tRNA | Termination - when a stop codon reaches the A site & releases factor (protein) binds in A site which causes polypeptide to be released (by hydrolysis) |
| Silent Mutation | -A instead of G -no effect on amino acid sequence |
| Missense Mutation | -T instead of C -a wrong amino acid |
| Nonsense Mutation | -A instead of T -a stop codon where it shouldn't be |