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Testing Bacteria

TermDefinition
Catalase Test Catalase + enzyme destroys hydrogen peroxide
Sugar Fermentation Test inverted tube captures CO2 if it's produced, also indicator (phenol red) turns yellow in presence of acid and magenta in presence of ammonia/base
Oxidase Test Tests for presence of cytochrome oxidase (e- transport chain); turns purple if cytochrome oxidase is present; a strict anaerobe is oxidase -
Indole Test Deamination of tryptophan to indole; media rich in tryptophan; E coli is one of the few indole + enterics
KIA/ H2S Production Thiosulfate reductase catalyzes sulfur to H2S gas, reacts with FeS to form black ppt. KIA has iron/sugars too; bubbles and cracks form if gas is made
Urease Test Catalyzes the breakdown of urea to ammonia and bicarb; pink- basic, yellow otherwise
Vogues-Proskauer Test Positive if pyruvate is fermented into butanediol and CO2
Citrate Test Tests if citrate can be used as energy source, E. coli is negative and Klebsiella is positive
Created by: esims2
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