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Lab Quiz 5-8

What are the three different carbs tested for in the Phenol Red Broth test? Glucose, Lactose, and Sucrose.
How to interpret the results of a Phenol Red Broth test (card 1 of 2) Yellow broth with bubble in tube = Fermentation with acid and gad end products Yellow broth with no bubble in tube = Fermentation with acid end products but no gas
How to interpret the results of a Phenol Red Broth test (card 2 of 2) Red Broth with no bubble in tube = No fermentation Pink Broth with no bubble in tube = Degradation of peptone, alkaline end products
Which enzymes must microbes produce to break down either lactose or sucrose? To break down lactose - B-galctosidase To break down sucrose - Sucrase
What is Methyl Red Used for? Detecting whether or not an organism can conduct mixed acid fermentation
What is Voges-Proskauer used for? Detecting organisms that utilize butylene glycol to produce acetoin
What are some examples of mixed acid fermentation byproducts? Lactic Acid Acetic Acid Formic Acid
What reagents used to produce a color change in a methyl red test? Methyl Red
What reagents are used to produce a color change in a Voges-Proskauer test? Barritt’s A and Barritt’s B reagent
How do you interpret the results of a methyl red test? Red result means positive for mixed acid fermentation, no color change means no mixed acid fermentation
How do you interpret the results of a Voges-Proskauer test? Red result means acetoin production, no color change means no acetoin has been produced
What is the purpose of a citrate test? To find out whether or not an organism can utilize citrate as a carbon source
What reagent is used in a citrate test and what are the byproducts? Reagent - Sodium citrate, has byproducts oxaloacetate & pyruvate.
What is an oxidase test used for? Oxidase is used to identify organisms that produce the enzyme cytochrome oxidase.
What is a catalse test used for? Catalase is used to identify cells that can break down hydrogen peroxide into H2O and O2.
In a positive oxidase test what enzymes are produced? A positive oxidase test produces cytochrome oxidase.
In a positive catalase taste what enzymes are produced? A positive catalase test produces catalase.
What is the cytochrome oxidase enzyme used for? Cytochrome oxidase is used in the electron transport chain as a donor transfer electrons to oxygen
What is the catalase enzyme used for? Catalase is used to get rid of peroxide in an O2 environment
What does a positive catalase test look like? A positive catalase test produces bubbles because of the evolution of O2 gas.
How is a positive oxidase reaction determined? A positive oxidase reaction will turn blue or purple within 15 seconds
How is a positive catalase reaction determined? A positive catalase reaction is determined by bubbling.
What does a positive oxidase test look like? An oxidase positive will turn violet to purple immediately or within 10 to 30 seconds. Delayed reactions should be ignored.
Created by: skline1982



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