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Lab Quiz 5-8
| Term | Definition |
|---|---|
| What are the three different carbs tested for in the Phenol Red Broth test? | Glucose, Lactose, and Sucrose. |
| How to interpret the results of a Phenol Red Broth test (card 1 of 2) | Yellow broth with bubble in tube = Fermentation with acid and gad end products Yellow broth with no bubble in tube = Fermentation with acid end products but no gas |
| How to interpret the results of a Phenol Red Broth test (card 2 of 2) | Red Broth with no bubble in tube = No fermentation Pink Broth with no bubble in tube = Degradation of peptone, alkaline end products |
| Which enzymes must microbes produce to break down either lactose or sucrose? | To break down lactose - B-galctosidase To break down sucrose - Sucrase |
| What is Methyl Red Used for? | Detecting whether or not an organism can conduct mixed acid fermentation |
| What is Voges-Proskauer used for? | Detecting organisms that utilize butylene glycol to produce acetoin |
| What are some examples of mixed acid fermentation byproducts? | Lactic Acid Acetic Acid Formic Acid |
| What reagents used to produce a color change in a methyl red test? | Methyl Red |
| What reagents are used to produce a color change in a Voges-Proskauer test? | Barritt’s A and Barritt’s B reagent |
| How do you interpret the results of a methyl red test? | Red result means positive for mixed acid fermentation, no color change means no mixed acid fermentation |
| How do you interpret the results of a Voges-Proskauer test? | Red result means acetoin production, no color change means no acetoin has been produced |
| What is the purpose of a citrate test? | To find out whether or not an organism can utilize citrate as a carbon source |
| What reagent is used in a citrate test and what are the byproducts? | Reagent - Sodium citrate, has byproducts oxaloacetate & pyruvate. |
| What is an oxidase test used for? | Oxidase is used to identify organisms that produce the enzyme cytochrome oxidase. |
| What is a catalse test used for? | Catalase is used to identify cells that can break down hydrogen peroxide into H2O and O2. |
| In a positive oxidase test what enzymes are produced? | A positive oxidase test produces cytochrome oxidase. |
| In a positive catalase taste what enzymes are produced? | A positive catalase test produces catalase. |
| What is the cytochrome oxidase enzyme used for? | Cytochrome oxidase is used in the electron transport chain as a donor transfer electrons to oxygen |
| What is the catalase enzyme used for? | Catalase is used to get rid of peroxide in an O2 environment |
| What does a positive catalase test look like? | A positive catalase test produces bubbles because of the evolution of O2 gas. |
| How is a positive oxidase reaction determined? | A positive oxidase reaction will turn blue or purple within 15 seconds |
| How is a positive catalase reaction determined? | A positive catalase reaction is determined by bubbling. |
| What does a positive oxidase test look like? | An oxidase positive will turn violet to purple immediately or within 10 to 30 seconds. Delayed reactions should be ignored. |
Created by:
skline1982
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