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Bio 1 CH 20
| Question | Answer |
|---|---|
| why is it important to assess sea bass population stocks? | -member of the ecosystem -commercial fishery -recreational fishery |
| what gene did scientists use in the sea bass research? | ribosomal gene, oligonucleotides |
| what part of the gene was used for gene sequencing? | noncoding spacer region of the ribosomal gene |
| what part of the gene was used to restriction fragment length polymorphism analysis (RFLP)? | oligonucleotides |
| how did scientists locate, isolate, and amplify the noncoding spacer unit of the ribosomal gene? | polymerase chain reaction (PCR) |
| describe PCR | -first the DNA genome is heated to denature the double strand into two single strands |
| what role did gel electrophoresis play in gene sequencing? | DNA can be extracted from the gel |
| compare PCR with DNA sequencing | PCR is cheaper and DNA sequencing (golden standard) is expensive |
| why are dideoxy nucleotide triphosphates used in gene sequencing and how do they enable DNA to be sequenced? | to sequence the ribosomal gene spacer unit |
| what is highly throughput technology and bioinformatics? | DNA sequencing, expensive, most labs will send off their samples for sequencing |
| describe RFLP | use entire ribosome gene, inexpensive -cycle 1 yeilds 2 molecules -cycle 2 yields 4 molecules -cycle 3 yields 8 molecules; 2 molecules match target sequence |
| describe short tendem repeating units are | -paternity test, f -or a male offspring look at Y chromosome -for a female offspring look at a series of sequences -maternity test, look at the mitochondrial DNA |
Created by:
KAzetapi
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