BZ 310- Lab Quiz 3 Word Scramble
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Question | Answer |
what was plasmid 1? | pBS |
what was plasmid 2? | pUC7K |
two types of gel for electrophoresis | poly-acrylamide and agarose |
what makes DNA visible on gels? | ethidium bromide |
how to make cells competent? (2) | heat or CaCl2 |
what did plasmid 1 grow on and not grow on? | grew on ampicillin, not kanamycin (pBS) |
what did plasmid 2 grow on? | both kanamycin and ampicillin (pUC7K) |
CFU/mL | # colonies per mL plated / dilution (1/10 etc) |
how to find g/plasmid | 700 g/mol (1 base pair) x # base pairs / 6.022E23 |
fraction transformed | CFU/mL transformed / CFU/mL control |
transformation efficiency | CFU/mL / ug plasmid added |
what makes glycerinated myofibers contract? | ATP, Mg, Ca, K |
what is added to observe skeletal muscle and why? | glycerin, removed membrane but leaves contractile apparatus intact |
what did we add to sperm and why? | Triton X-100 to removes membranes, DTT stabilize ATP binding site |
overall photosynthesis reaction | 6CO2 + 12H2O -> C6H12O6 + 6O2 + 6H2O |
where do the oxygens in O2 product of photosynthesis come from? | the H2O |
what color was DCIP when reduced? | clear |
what color was DCIP when oxidized? | blue |
DCIP | in place of NADPH for hill reaction |
order of hill reaction | PS II, PQ, PS I, ferredoxin, NADPH |
where are cells coming from this week? | SF9 insect cells (fall armyworm) |
what will be used this week to prevent freezing damage? | DMSO |
how will we make a sterile environment this week? | UV |
what will we use this week to prevent contamination? | flow cabinets |
each muscle cell is a | fiber |
the striated appearance of muscle fibers is caused by | actin and myosin overlap |
photosynthesis requires light, H2O, CO2, and | chloroplasts |
why use insect cells? | less sensitive to temperature and CO2 concentration |
Created by:
melaniebeale
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