Special Satins Word Scramble
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Term | Definition |
Giesma for H. Pylori | stains for helicobacter pylori diff quick solution I diff quick solution II Acetic water, then check with microscope |
Giesma for H. Pylori | H. pylori.................DARK BLUE Other bacteria.......BLUE Nuclei....................DARK BLUE Cytoplasm...............PINK |
Brown-Hopps Modified Gram Stain | stains gram positive and negative bacteria crystal violet (gram positive) gram iodine(mordant) blot paper, acetone (decolorize neg) basic fuchsin->gallego->acetone->picric acid acetone |
Brown-Hopps Modified Gram Stain | Gram (+).........BLUE Gram(-)...........RED Background.....YELLOW Nuclei..............LIGHT RED |
Auramine-Rhodamine Fleorescene | stains for Mycobacterium Tuberculosis or other AFB Auromine-Rhodamnie B solution-> acid alcohol-> Eriochrome Black T |
Auramine-Rhodamine Fleorescene | AFB..................reddish yellow fluorescene Background......BLACK |
Fite Acid Fast Stain for Leprosy | demonstrates mycobacterium leprae xylene with peanut oil-> ZN carbol fuchsin-> acid alcohol-> methyl blue |
Fite Acid Fast Stain for Leprosy | M leprae...............RED Background...........BLUE |
Acid Fast Kinyoun Acid Fast Stain | Kinyoun Carbol-Fuchsin -> Acid Alcohol (to differentiate)-> Methylene Blue (counterstain) AFB....................BRIGHT RED Background.........BLUE |
Ziehl-Neelsen for AFB | Ziehl Neelson Carbol Fuchsin (uses ethyl alcohol) acid alcohol-> methylene blue AFB....................BRIGHT RED Background.........BLUE |
Hotchkiss-Mcmanus PAS for Fungus | demonstrates fungus Fungi.................ROSE Background.......GREEN |
FUNGUS STAINS | PASF CHROMIC ACID-SCHIFF STAIN GRIDLEY GROCOTT METHANAMINE SILVER NITRATE |
Grocott Methanamine- Silver Nitrate Fungus Stain | chromic acid is the oxidizer for the fungal cell wall stains for pneumocystis jirovecii chromic acid->sodium bisulfate-> pre-heated methanamine silver-> gold chloride-> sodium thiosulfate -> light green |
Grocott Methanamine- Silver Nitrate Fungus Stain | Fungi......................BLACK Mucin......................TAUPE Background..............GREEN |
SPIROCHETE STAINS | WARTHIN-STARRY DIETERLE STEINER & STEINER |
Warthin-Starry for Spirochetes | 2% Silver nitrate (developer) 1% Silver nitrate (impregnation) Developer (gelatin, hydroquinine, 2% silver nitrate) |
Warthin-Starry for Spirochetes | Spirochetes & bacteria.........BLACK Background..........................YELLOW/BROWN |
Steiner | demonstrates spirochetes, helicobacter, and legionella uses gum mastic |
Steiner | Spirochetes, H. pylori, L. Pneumophila, other nonfilamentous bacteria...............DARK BROWN TO BLACK Background............................................LIGHT YELLOW |
Created by:
burkan2
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