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Special Satins

Microorganisms

TermDefinition
Giesma for H. Pylori stains for helicobacter pylori diff quick solution I diff quick solution II Acetic water, then check with microscope
Giesma for H. Pylori H. pylori.................DARK BLUE Other bacteria.......BLUE Nuclei....................DARK BLUE Cytoplasm...............PINK
Brown-Hopps Modified Gram Stain stains gram positive and negative bacteria crystal violet (gram positive) gram iodine(mordant) blot paper, acetone (decolorize neg) basic fuchsin->gallego->acetone->picric acid acetone
Brown-Hopps Modified Gram Stain Gram (+).........BLUE Gram(-)...........RED Background.....YELLOW Nuclei..............LIGHT RED
Auramine-Rhodamine Fleorescene stains for Mycobacterium Tuberculosis or other AFB Auromine-Rhodamnie B solution-> acid alcohol-> Eriochrome Black T
Auramine-Rhodamine Fleorescene AFB..................reddish yellow fluorescene Background......BLACK
Fite Acid Fast Stain for Leprosy demonstrates mycobacterium leprae xylene with peanut oil-> ZN carbol fuchsin-> acid alcohol-> methyl blue
Fite Acid Fast Stain for Leprosy M leprae...............RED Background...........BLUE
Acid Fast Kinyoun Acid Fast Stain Kinyoun Carbol-Fuchsin -> Acid Alcohol (to differentiate)-> Methylene Blue (counterstain) AFB....................BRIGHT RED Background.........BLUE
Ziehl-Neelsen for AFB Ziehl Neelson Carbol Fuchsin (uses ethyl alcohol) acid alcohol-> methylene blue AFB....................BRIGHT RED Background.........BLUE
Hotchkiss-Mcmanus PAS for Fungus demonstrates fungus Fungi.................ROSE Background.......GREEN
FUNGUS STAINS PASF CHROMIC ACID-SCHIFF STAIN GRIDLEY GROCOTT METHANAMINE SILVER NITRATE
Grocott Methanamine- Silver Nitrate Fungus Stain chromic acid is the oxidizer for the fungal cell wall stains for pneumocystis jirovecii chromic acid->sodium bisulfate-> pre-heated methanamine silver-> gold chloride-> sodium thiosulfate -> light green
Grocott Methanamine- Silver Nitrate Fungus Stain Fungi......................BLACK Mucin......................TAUPE Background..............GREEN
SPIROCHETE STAINS WARTHIN-STARRY DIETERLE STEINER & STEINER
Warthin-Starry for Spirochetes 2% Silver nitrate (developer) 1% Silver nitrate (impregnation) Developer (gelatin, hydroquinine, 2% silver nitrate)
Warthin-Starry for Spirochetes Spirochetes & bacteria.........BLACK Background..........................YELLOW/BROWN
Steiner demonstrates spirochetes, helicobacter, and legionella uses gum mastic
Steiner Spirochetes, H. pylori, L. Pneumophila, other nonfilamentous bacteria...............DARK BROWN TO BLACK Background............................................LIGHT YELLOW
Created by: burkan2