Micro 204 pour plate lab 9
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
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| A freshly poured plate should be rotated carefully in a | figure 8
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| What is the predominant shape of subsurface colonies | lens shape
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| Which method of separating organisms seems to achieve the best separation? | pour plate
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| Give 2 reasons why the nutrient agar must be cooled to 45 degrees C before inoculation and pouring. | Dilute to not kill the organism,
Lesson the amount of condensation
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| Name the instrument used to help count colonies on plates is the X X X. | Quebec colony counter
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| This technique is said to be roughly quantitative because the loop used contains only approximately X ml. | .01 ml
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| If your tubes solidify after inoculation but before you pour them into plates, what happens to the bacteria in the tubes when you remelt them? | The organism dies.
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| Give one reason why you should avoid slopping agar on the cover while mixing the sample. | Contaminate the environment, Invalid colony count
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| When you incubate these plated the cover should be X. | on the bottom
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| When pouring an agar plate remove the cover and lay it on the bench. G or B | Bad
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| Hold the cover up just enough to admit the neck of the flask. G or B | Good
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| Flame the neck of the agar flask before pouring the plate. G or B | Good
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| As soon as the tube is inoculated in this exercise, remove the cap and immediately pour the plate. G or B | Bad, you need to roll the tube to allow the organism to fill the entire tube.
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| heat the lip of the tube so that the agar sizzles as it is being poured into the plate | Bad
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| Incubate your plates with their covers facing up | Bad
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| After streaking a plate with a pure culture, lay the loop down on the bench | Bad
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| Carefully remove the cap on a tube, and without setting it down make your transfer. | Good
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| Flame your inoculation loop before and after the transfer | Good
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| While attempting to remove a tube cap, your loop accidentally touches the sleeve of your lab coat. | Bad
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Created by:
kmorris8
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