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Micro pour plate 9

Micro 204 pour plate lab 9

QuestionAnswer
A freshly poured plate should be rotated carefully in a figure 8
What is the predominant shape of subsurface colonies lens shape
Which method of separating organisms seems to achieve the best separation? pour plate
Give 2 reasons why the nutrient agar must be cooled to 45 degrees C before inoculation and pouring. Dilute to not kill the organism, Lesson the amount of condensation
Name the instrument used to help count colonies on plates is the X X X. Quebec colony counter
This technique is said to be roughly quantitative because the loop used contains only approximately X ml. .01 ml
If your tubes solidify after inoculation but before you pour them into plates, what happens to the bacteria in the tubes when you remelt them? The organism dies.
Give one reason why you should avoid slopping agar on the cover while mixing the sample. Contaminate the environment, Invalid colony count
When you incubate these plated the cover should be X. on the bottom
When pouring an agar plate remove the cover and lay it on the bench. G or B Bad
Hold the cover up just enough to admit the neck of the flask. G or B Good
Flame the neck of the agar flask before pouring the plate. G or B Good
As soon as the tube is inoculated in this exercise, remove the cap and immediately pour the plate. G or B Bad, you need to roll the tube to allow the organism to fill the entire tube.
heat the lip of the tube so that the agar sizzles as it is being poured into the plate Bad
Incubate your plates with their covers facing up Bad
After streaking a plate with a pure culture, lay the loop down on the bench Bad
Carefully remove the cap on a tube, and without setting it down make your transfer. Good
Flame your inoculation loop before and after the transfer Good
While attempting to remove a tube cap, your loop accidentally touches the sleeve of your lab coat. Bad
Created by: kmorris8
 

 



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