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exam 1 subjects

Quiz yourself by thinking what should be in each of the black spaces below before clicking on it to display the answer.
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Question
Answer
what is a specialized element?   capsid  
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viruses are best observed by.....   TEM  
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what kind of staining is used in TEM   negative staining  
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viral envelope is composed of...   lipid membrane and viral protein embedded  
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a nucleocapsid is...   a naked virus  
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how many structual types of viruses are there?   1. icosahedral 2. helical 3. complex  
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envoloped virus consist of...   nucleocapsid and envelope  
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smallest virus is a...   parovirus  
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genus = ?   virus  
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family = ?   viridae  
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order = ?   virales  
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isosahedron is...   20 sides, equilateral triangles on each side, 12 corners, and 30 edges... similar to a sphere.  
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2 fold symmetry for a icosahedron is...   can rotate at 180 degrees and have the same orientation from the side edge  
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3 fold symmetry for a icosahedron is...   through the middle has equal symmetry  
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5 fold symmetry for a icosahedron is...   folding through a corner  
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intracapsomeric bond and intercapsomeric bonds are what kind of bonding?   non covalent bonds  
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which one is stronger bonding? inter or intra- caposmeric?   intracapsomeric  
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an example of a complex virus structure   poxvirus  
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what determines virus structure?   protein protein interaction, protein and NA interaction, protein structure, protein sequence, viral gene sequence  
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virus that contain GLUE (matrix protiens)   orthoyxoviridae, pamyxovirdae, rhabdoviridae  
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what type of viruses are sensitive to bile and acid?   enveloped viruses  
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ebola virus   filoviridae  
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rabies virus   rhabdoviridae  
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rota virus   reoviridae  
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common cold   rhinoviruses,adenoviridae, picornaviridae  
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rubella   togaviridae  
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yellow fever and west nile virus   flaviviridae  
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SARS   coronaviridae  
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Influenza   orthomyxoviridae  
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polio   picornaviridae  
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measles   paramyxoviridae  
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chickenpox, herpes, EBV   herpesviridae  
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hanta virus   bunyaviridae  
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lassa fever   arenaviridae  
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primary culture is...   1st set of cells directly frm a animal and cultured and cells are growing  
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primary cell line is...   primary culture being subcultured that are diploid  
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continuous cell line...   ready supply of immortal aneuploid cells  
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types of assay...   infectivity and physico-chemical  
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types of infectivity assay...   quantal and quantitative  
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quantal assay consist of...   recording the highest dilution that is causing CPE  
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a colony represents a....   specific strain; pure culture  
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one hit kinetics   one infectious virus is suffient to form a plaque  
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two hit kinetics   2 virus particles is suffient to form a plaque  
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particle to infectivity ratio (PI)...   total # of particles per infectious sites  
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Efficiency of plating (EOP)   # of infectious units per total # of particle  
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multiplicity of infection (MOI)   m = -ln P(o)  
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one step growth expt is done by   max dulbruck  
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