exam 1 subjects
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
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what is a specialized element? | capsid
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viruses are best observed by..... | TEM
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what kind of staining is used in TEM | negative staining
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viral envelope is composed of... | lipid membrane and viral protein embedded
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a nucleocapsid is... | a naked virus
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how many structual types of viruses are there? | 1. icosahedral
2. helical
3. complex
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envoloped virus consist of... | nucleocapsid and envelope
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smallest virus is a... | parovirus
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genus = ? | virus
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family = ? | viridae
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order = ? | virales
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isosahedron is... | 20 sides, equilateral triangles on each side, 12 corners, and 30 edges... similar to a sphere.
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2 fold symmetry for a icosahedron is... | can rotate at 180 degrees and have the same orientation from the side edge
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3 fold symmetry for a icosahedron is... | through the middle has equal symmetry
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5 fold symmetry for a icosahedron is... | folding through a corner
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intracapsomeric bond and intercapsomeric bonds are what kind of bonding? | non covalent bonds
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which one is stronger bonding? inter or intra- caposmeric? | intracapsomeric
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an example of a complex virus structure | poxvirus
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what determines virus structure? | protein protein interaction, protein and NA interaction, protein structure, protein sequence, viral gene sequence
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virus that contain GLUE (matrix protiens) | orthoyxoviridae, pamyxovirdae, rhabdoviridae
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what type of viruses are sensitive to bile and acid? | enveloped viruses
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ebola virus | filoviridae
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rabies virus | rhabdoviridae
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rota virus | reoviridae
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common cold | rhinoviruses,adenoviridae, picornaviridae
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rubella | togaviridae
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yellow fever and west nile virus | flaviviridae
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SARS | coronaviridae
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Influenza | orthomyxoviridae
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polio | picornaviridae
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measles | paramyxoviridae
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chickenpox, herpes, EBV | herpesviridae
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hanta virus | bunyaviridae
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lassa fever | arenaviridae
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primary culture is... | 1st set of cells directly frm a animal and cultured and cells are growing
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primary cell line is... | primary culture being subcultured that are diploid
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continuous cell line... | ready supply of immortal aneuploid cells
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types of assay... | infectivity and physico-chemical
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types of infectivity assay... | quantal and quantitative
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quantal assay consist of... | recording the highest dilution that is causing CPE
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a colony represents a.... | specific strain; pure culture
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one hit kinetics | one infectious virus is suffient to form a plaque
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two hit kinetics | 2 virus particles is suffient to form a plaque
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particle to infectivity ratio (PI)... | total # of particles per infectious sites
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Efficiency of plating (EOP) | # of infectious units per total # of particle
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multiplicity of infection (MOI) | m = -ln P(o)
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one step growth expt is done by | max dulbruck
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