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virology ex1
exam 1 subjects
| Question | Answer |
|---|---|
| what is a specialized element? | capsid |
| viruses are best observed by..... | TEM |
| what kind of staining is used in TEM | negative staining |
| viral envelope is composed of... | lipid membrane and viral protein embedded |
| a nucleocapsid is... | a naked virus |
| how many structual types of viruses are there? | 1. icosahedral 2. helical 3. complex |
| envoloped virus consist of... | nucleocapsid and envelope |
| smallest virus is a... | parovirus |
| genus = ? | virus |
| family = ? | viridae |
| order = ? | virales |
| isosahedron is... | 20 sides, equilateral triangles on each side, 12 corners, and 30 edges... similar to a sphere. |
| 2 fold symmetry for a icosahedron is... | can rotate at 180 degrees and have the same orientation from the side edge |
| 3 fold symmetry for a icosahedron is... | through the middle has equal symmetry |
| 5 fold symmetry for a icosahedron is... | folding through a corner |
| intracapsomeric bond and intercapsomeric bonds are what kind of bonding? | non covalent bonds |
| which one is stronger bonding? inter or intra- caposmeric? | intracapsomeric |
| an example of a complex virus structure | poxvirus |
| what determines virus structure? | protein protein interaction, protein and NA interaction, protein structure, protein sequence, viral gene sequence |
| virus that contain GLUE (matrix protiens) | orthoyxoviridae, pamyxovirdae, rhabdoviridae |
| what type of viruses are sensitive to bile and acid? | enveloped viruses |
| ebola virus | filoviridae |
| rabies virus | rhabdoviridae |
| rota virus | reoviridae |
| common cold | rhinoviruses,adenoviridae, picornaviridae |
| rubella | togaviridae |
| yellow fever and west nile virus | flaviviridae |
| SARS | coronaviridae |
| Influenza | orthomyxoviridae |
| polio | picornaviridae |
| measles | paramyxoviridae |
| chickenpox, herpes, EBV | herpesviridae |
| hanta virus | bunyaviridae |
| lassa fever | arenaviridae |
| primary culture is... | 1st set of cells directly frm a animal and cultured and cells are growing |
| primary cell line is... | primary culture being subcultured that are diploid |
| continuous cell line... | ready supply of immortal aneuploid cells |
| types of assay... | infectivity and physico-chemical |
| types of infectivity assay... | quantal and quantitative |
| quantal assay consist of... | recording the highest dilution that is causing CPE |
| a colony represents a.... | specific strain; pure culture |
| one hit kinetics | one infectious virus is suffient to form a plaque |
| two hit kinetics | 2 virus particles is suffient to form a plaque |
| particle to infectivity ratio (PI)... | total # of particles per infectious sites |
| Efficiency of plating (EOP) | # of infectious units per total # of particle |
| multiplicity of infection (MOI) | m = -ln P(o) |
| one step growth expt is done by | max dulbruck |
Created by:
ibarguenl17
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